Systems & Computational Biology
- Missense variant interaction scanning reveals a critical role of the FERM domain for tumor suppressor protein NF2 conformation and function
Deep mutational interaction perturbation scanning of NF2 with conformation-dependent interaction partners KDM1A, EMILIN, and PIK3R3 revealed two regions critical for NF2 tumor suppressor function.
- A new metabolic model of Drosophila melanogaster and the integrative analysis of Parkinson’s disease
The reconstruction of a comprehensive and literature-curated Drosophila metabolic model, iDrosophila1, provided a powerful platform for studying metabolism with systems medicine perspective.
- Evaluation of genetic demultiplexing of single-cell sequencing data from model species
The authors demonstrate that scRNA-seq sample pooling followed by genetics-based separation of individuals is an effective means to identify individual samples in a variety of commonly studied species.
- Antibody reliability influences observed mRNA–protein correlations in tumour samples
Upadhya and Ryan show that the reliability of antibodies used for RPPA profiling influences the mRNA-protein correlations observed in tumour cohorts.
- Predictive data-driven modeling of C-terminal tyrosine function in the EGFR signaling network
The site-specific function of EGFR’s C-terminal tyrosines is interrogated by phosphotyrosine phosphoproteomics to build a data-driven model that links cell response to signaling nodes and elucidates novel associations in the EGFR signaling network.
- Z-flipon variants reveal the many roles of Z-DNA and Z-RNA in health and disease
Z-DNABERT uses the transformer algorithm to map genomic Z-DNA. We validate the approach using chemical footprinting data from live cells. We show the utility of the approach by mapping the Z-flipons discovered to Mendelian disease variants and various quantitative traits.
- Gapless provides combined scaffolding, gap filling, and assembly correction with long reads
gapless is a new tool for combined execution of correction, scaffolding and gap filling using PacBio or Oxford Nanopore reads.
- Proteomic analysis reveals microvesicles containing NAMPT as mediators of radioresistance in glioma
Glioma patients often relapse because of resistance to radiation therapy. The transfer of the metabolic enzyme NAMPT via microvesicles and the accompanying elevated levels of NAD are found to be a mechanism by which glioma cells become resistant to radiation.
- Analysis of context-specific KRAS–effector (sub)complexes in Caco-2 cells
We used affinity purification of KRAS in different genetic and growth contexts to determine KRAS-mediated complexes, their rewiring in specific contexts, and their link to functional outcomes.
- RAS and PP2A activities converge on epigenetic gene regulation
This study reveals a hidden regulatory layer in epigenetic gene expression in cancer by co-operative phosphoregulation by RAS and tumor suppressor phosphatase PP2A.