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Synthetic Biology

  • Optogenetic control of pheromone gradients and mating behavior in budding yeast
    Open Access
    Optogenetic control of pheromone gradients and mating behavior in budding yeast

    Alvaro Banderas, Maud Hofmann, Céline Cordier, Matthias Le Bec, M Carolina Elizondo-Cantú, Lionel Chiron, Sylvain Pouzet, Yotam Lifschytz, Wencheng Ji, Ariel Amir, Vittore Scolari, Pascal Hersen

    Alvaro Banderas ... Pascal Hersen

    Published 11 April 2025

    The authors combined theory and experiment to develop light-induced yeast pheromone gradients that spatially control growth, mating and morphology in hydrogel-embedded cells.

  • Concatemer-assisted stoichiometry analysis: targeted mass spectrometry for protein quantification
    Open Access
    Concatemer-assisted stoichiometry analysis: targeted mass spectrometry for protein quantification

    Jiaxi Cai, Yun Quan, Cindy Yuxuan Zhang, Ziyi Wang, Stephen M Hinshaw, Huilin Zhou, Raymond T Suhandynata

    Jiaxi Cai ... Raymond T Suhandynata

    Published 31 December 2024

    Concatemer-assisted stoichiometry analysis (CASA) addresses a common challenge in cell biology: absolute quantification of protein subunits in native protein complexes.

  • In vivo probing of SECIS-dependent selenocysteine translation in Archaea
    Open Access
    In vivo probing of SECIS-dependent selenocysteine translation in Archaea

    Nils Peiter, Michael Rother

    Nils Peiter ... Michael Rother

    Published 31 October 2022

    By turning a bacterial reporter into an archaeal selenoprotein, in vivo probing of structure–function relations during UGA recoding for selenoprotein synthesis in Archaea is greatly facilitated.

  • Synthetic evolution of herbicide resistance using a T7 RNAP–based random DNA base editor
    Open Access
    Synthetic evolution of herbicide resistance using a T7 RNAP–based random DNA base editor

    Haroon Butt, Jose Luis Moreno Ramirez, Magdy Mahfouz

    Haroon Butt ... Magdy Mahfouz

    Published 28 September 2022

    A chimeric fusion of T7 RNAP and deaminase edits the DNA under the T7 promoter in plant cells. It directs the continuous synthetic evolution of OsALS to produce variants with herbicide resistance.

  • Sufficiency analysis of estrogen responsive enhancers using synthetic activators
    Open Access
    Sufficiency analysis of estrogen responsive enhancers using synthetic activators

    Matthew Ginley-Hidinger, Julia B Carleton, Adriana C Rodriguez, Kristofer C Berrett, Jason Gertz

    Matthew Ginley-Hidinger ... Jason Gertz

    Published 30 September 2019

    Enhancers bound by synthetic activators can recreate a transcriptional response to estrogen, drive different levels of gene expression, and work independently to regulate transcription.

  • Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements
    Open Access
    Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements

    Verena Thormann, Laura V Glaser, Maika C Rothkegel, Marina Borschiwer, Melissa Bothe, Alisa Fuchs, Sebastiaan H Meijsing

    Verena Thormann ... Sebastiaan H Meijsing

    Published 13 March 2019

    This study shows that addition of a single transcription factor binding site can be sufficient to convert a gene into a glucocorticoid receptor target.

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