Synthetic Biology
Optogenetic control of pheromone gradients and mating behavior in budding yeastThe authors combined theory and experiment to develop light-induced yeast pheromone gradients that spatially control growth, mating and morphology in hydrogel-embedded cells.
Concatemer-assisted stoichiometry analysis: targeted mass spectrometry for protein quantificationConcatemer-assisted stoichiometry analysis (CASA) addresses a common challenge in cell biology: absolute quantification of protein subunits in native protein complexes.
In vivo probing of SECIS-dependent selenocysteine translation in ArchaeaBy turning a bacterial reporter into an archaeal selenoprotein, in vivo probing of structure–function relations during UGA recoding for selenoprotein synthesis in Archaea is greatly facilitated.
Synthetic evolution of herbicide resistance using a T7 RNAP–based random DNA base editorA chimeric fusion of T7 RNAP and deaminase edits the DNA under the T7 promoter in plant cells. It directs the continuous synthetic evolution of OsALS to produce variants with herbicide resistance.
Sufficiency analysis of estrogen responsive enhancers using synthetic activatorsEnhancers bound by synthetic activators can recreate a transcriptional response to estrogen, drive different levels of gene expression, and work independently to regulate transcription.
Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elementsThis study shows that addition of a single transcription factor binding site can be sufficient to convert a gene into a glucocorticoid receptor target.