Structural Biology
Stoichiometry of Rtt109 complexes with Vps75 and histones H3-H4The work determines the relative and absolute stoichiometry of a 5-chain protein complex involved in histone chaperoning and acetylation. Using sedimentation velocity and light scattering, it uncovers a dynamic equilibrium of complex self-association.
CryoEM structure of Drosophila flight muscle thick filaments at 7 Å resolutionRelaxed thick filaments from Drosophila flight muscle show disordered myosin heads, a ribbon arrangement of myosin tails and a novel protein, stretchin-klp, on the filament surface.
Structure of human Dispatched-1 provides insights into Hedgehog ligand biogenesisA cryo-EM structure of human Dispatched-1 reveals a unique open conformation of its extracellular domains for Hedgehog ligand binding.
Structure of avian influenza hemagglutinin in complex with a small molecule entry inhibitorThe binding properties of fusion inhibitor CBS1117 to hemagglutinin by x-ray crystallography, NMR, and mutagenesis give insights into mechanism and guidance for chemical optimization.
STAG1 vulnerabilities for exploiting cohesin synthetic lethality in STAG2-deficient cancersThis work highlights the degradation of STAG1 and inhibition of its interaction with cohesin as promising therapeutic strategies for exploiting synthetic lethality to treat STAG2-mutated tumors and lays the groundwork for the discovery of STAG1-directed small molecules.
Structural basis of HapEP88L-linked antifungal triazole resistance in Aspergillus fumigatusThe mutation P88L in subunit HapE of the CCAAT transcription factor causes resistance of Aspergillus fumigatus to azole drugs due to altered binding, bending, and transcription of target promoters.
Structures of SALSA/DMBT1 SRCR domains reveal the conserved ligand-binding mechanism of the ancient SRCR foldThe structures of SALSA SRCR domains 1 and 8 reveal a cation-dependent mechanism for ligand recognition, contributing to important roles in the immune system and cellular signalling. The cation-binding sites are conserved across all SRCR domains, suggesting conserved functional mechanisms.
A key region of molecular specificity orchestrates unique ephrin-B1 utilization by Cedar virusAn expanded hydrophobic cavity within the structurally constrained receptor-binding site of the Cedar virus attachment glycoprotein facilitates idiosyncratic utilization of ephrin-B1.
Structural and functional characterization of the mitochondrial complex IV assembly factor Coa6The structures of the mitochondrial complex IV assembly factor Coa6 and a pathogenic mutant variant (W59CCoa6) are reported, providing molecular mechanisms for its mode of action and the loss-of-function mutation.
KAP1 is an antiparallel dimer with a functional asymmetryThis study reveals the architecture of human KAP1 by integrating molecular modeling with small-angle X-ray scattering and single-molecule experiments. KAP1 dimers feature a structural asymmetry at the C-terminal domains that has functional implications for recruitment of HP1.