Structural Biology
Structural and functional characterization of the mitochondrial complex IV assembly factor Coa6The structures of the mitochondrial complex IV assembly factor Coa6 and a pathogenic mutant variant (W59CCoa6) are reported, providing molecular mechanisms for its mode of action and the loss-of-function mutation.
KAP1 is an antiparallel dimer with a functional asymmetryThis study reveals the architecture of human KAP1 by integrating molecular modeling with small-angle X-ray scattering and single-molecule experiments. KAP1 dimers feature a structural asymmetry at the C-terminal domains that has functional implications for recruitment of HP1.
Subunit interactions and arrangements in the fission yeast Mis16–Mis18–Mis19 complexA structural study of the fission yeast Mis19 protein, required for centromere establishment, is presented together with data describing its interactions with functional partners Mis16 and Mis18.
Systematic identification of recognition motifs for the hub protein LC8LC8 is a eukaryotic hub protein that interacts with multifarious partners; analysis of more than 100 binding/nonbinding sequences led to an algorithm that predicts LC8 partners with 78% accuracy.
Assembly mechanisms of the bacterial cytoskeletal protein FilPFilP is a coiled-coil protein that self-assembles into striated filaments and meshwork that shares structural and biochemical characteristics with metazoan intermediate filaments.
ATP hydrolysis by KaiC promotes its KaiA binding in the cyanobacterial circadian clock systemATP hydrolysis in the KaiC hexamer triggers the exposure of its C-terminal segments into the solvent so as to capture KaiA, providing mechanistic insights into the circadian periodicity regulation.
Molecular characterization of CHAD domains as inorganic polyphosphate-binding modulesA domain of unknown function termed CHAD, present in all kingdoms of life, is characterized as a specific inorganic polyphosphate-binding domain.
Characterisation of class VI TRIM RING domains: linking RING activity to C-terminal domain identityThis study shows that members of the class VI TRIM E3 ligase family contain monomeric RING domains and display no ligase activity and may require additional regulatory events to promote ubiquitination.
Structural basis of guanine nucleotide exchange for Rab11 by SH3BP5The structure of the SH3BP5–Rab11a complex and structure-based mutational analyses reveal structural basis of nucleotide exchange for Rab11 by its specific guanine nucleotide exchange factor.
Folding–function relationship of the most common cystic fibrosis–causing CFTR conductance mutantsThe tight correlation between folding and function in cystic fibrosis patients with CFTR mutations of the altered-conductance CFTR class provides an attractive paradigm for characterizing mode of action of novel therapeutics.