Structural Biology
Molecular analysis and essentiality of Aro1 shikimate biosynthesis multi-enzyme in Candida albicansStructural characterization of shikimate pathway multi-enzyme Aro1 from opportunistic fungal pathogen Candida albicans provides the first molecular image of all five domains. Only four of these domains are active and individually essential for C. albicans viability.
Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26This study provides molecular insights into RNA target recognition by the TRIM-NHL protein Mei-P26 that functions in Drosophila ovarian germline stem cell maintenance, oogenesis, and spermatogenesis.
Structure and conformational dynamics of Clostridioides difficile toxin AThis study presents a complete structural model of TcdA holotoxin and sheds new lights into the conformational dynamics of TcdA and its roles in TcdA intoxication.
Structural basis of Nrd1–Nab3 heterodimerizationThe NMR structure of an Nrd1–Nab3 chimera describes the structural bases of Nrd1/Nab3 heterodimerization. Nrd1 embraces a bundle of helices in Nab3, building a large interface. Key mutations at that interface compromise cell fitness.
Structural basis of membrane recognition of Toxoplasma gondii vacuole by Irgb6Upon Toxoplasma gondii infection, Irgb6 is recruited to the parasitophorous vacuole membrane (PVM) where Irgb6 disrupts it. We solved the atomic structures of Irgb6 in two distinct nucleotide states, revealing a unique PVM-binding interface sensitive to GTPase cycling.
The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sitesPlant SYT1 transports lipids between endoplasmic reticulum and plasma membrane in response to stress. The protein flexibility and lipid-binding specificity determine SYT1 function at molecular level.
Dynamic changes in RNA–protein interactions and RNA secondary structure in mammalian erythropoiesisAnalysis of PIP-seq data from a model of erythropoiesis reveals dynamics of RBP–RNA interaction and changes in RNA secondary structure throughout red blood cell development.
Dynamic closed states of a ligand-gated ion channel captured by cryo-EM and simulationsCryo-EM structures and molecular simulations of a pentameric ligand-gated ion channel, GLIC, in resting and activating conditions implicate protonation sites and altered dynamics in sequential gating.
Crystal structure of inhibitor-bound human MSPL that can activate high pathogenic avian influenzaThe structure of extracellular domain of MSPL and inhibitor complex helps to understand the TTSP functions, including TMPRSS2, and provides the insights of the infection of influenza and SARS-CoV.
Structural comparison of GLUT1 to GLUT3 reveal transport regulation mechanism in sugar porter familyA new structure of GLUT1, compared with GLUT3, can explain substrate affinity differences, and provide a functional rationale for key structural motifs that define the universal Sugar Porter family.