Structural Biology
ATP hydrolysis by KaiC promotes its KaiA binding in the cyanobacterial circadian clock systemATP hydrolysis in the KaiC hexamer triggers the exposure of its C-terminal segments into the solvent so as to capture KaiA, providing mechanistic insights into the circadian periodicity regulation.
Molecular characterization of CHAD domains as inorganic polyphosphate-binding modulesA domain of unknown function termed CHAD, present in all kingdoms of life, is characterized as a specific inorganic polyphosphate-binding domain.
Characterisation of class VI TRIM RING domains: linking RING activity to C-terminal domain identityThis study shows that members of the class VI TRIM E3 ligase family contain monomeric RING domains and display no ligase activity and may require additional regulatory events to promote ubiquitination.
Structural basis of guanine nucleotide exchange for Rab11 by SH3BP5The structure of the SH3BP5–Rab11a complex and structure-based mutational analyses reveal structural basis of nucleotide exchange for Rab11 by its specific guanine nucleotide exchange factor.
Folding–function relationship of the most common cystic fibrosis–causing CFTR conductance mutantsThe tight correlation between folding and function in cystic fibrosis patients with CFTR mutations of the altered-conductance CFTR class provides an attractive paradigm for characterizing mode of action of novel therapeutics.
The crystal structure of Staufen1 in complex with a physiological RNA sheds light on substrate selectivityCombination of in vitro and in vivo data show that RNA sequence influences Staufen target recognition and that protein–RNA base contacts are required for Staufen function in Drosophila.
Specificity for deubiquitination of monoubiquitinated FANCD2 is driven by the N-terminus of USP1Deubiquitination of FANCD2, FANCI, and PCNA by USP1 is essential for DNA repair signalling. Reconstitution of the system reveals that USP1-mediated specificity towards K561 of FANCD2 is directed by a unique sequence at USP1's N-terminus.
Atomic force microscopy reveals structural variability amongst nuclear pore complexesStructural heterogeneity is resolved in isolated nuclear envelopes, revealing the lamina network and nuclear pore complexes of different sizes, as well as different morphologies in their transport barrier and different binding of nuclear transport receptors in the barrier.
Beetle luciferases with naturally red- and blue-shifted emissionNew crystal structures of red- and green blue–shifted beetle luciferases reveal that the color emission mechanism is dependent on the active site microenvironment affected by the conformation of loop regions.
Ribosome recycling is coordinated by processive events in two asymmetric ATP sites of ABCE1The stepwise ribosome disassembly in the translation cycle of eukaryotes and archaea is scheduled by discrete molecular events within the asymmetric ribosome recycling factor ABCE1.