Structural Biology
The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sitesPlant SYT1 transports lipids between endoplasmic reticulum and plasma membrane in response to stress. The protein flexibility and lipid-binding specificity determine SYT1 function at molecular level.
Dynamic changes in RNA–protein interactions and RNA secondary structure in mammalian erythropoiesisAnalysis of PIP-seq data from a model of erythropoiesis reveals dynamics of RBP–RNA interaction and changes in RNA secondary structure throughout red blood cell development.
Dynamic closed states of a ligand-gated ion channel captured by cryo-EM and simulationsCryo-EM structures and molecular simulations of a pentameric ligand-gated ion channel, GLIC, in resting and activating conditions implicate protonation sites and altered dynamics in sequential gating.
Crystal structure of inhibitor-bound human MSPL that can activate high pathogenic avian influenzaThe structure of extracellular domain of MSPL and inhibitor complex helps to understand the TTSP functions, including TMPRSS2, and provides the insights of the infection of influenza and SARS-CoV.
Structural comparison of GLUT1 to GLUT3 reveal transport regulation mechanism in sugar porter familyA new structure of GLUT1, compared with GLUT3, can explain substrate affinity differences, and provide a functional rationale for key structural motifs that define the universal Sugar Porter family.
Structure of the helicase core of Werner helicase, a key target in microsatellite instability cancersWRN is a key drug target for cancers with microsatellite instability. A crystal structure of the helicase domain of WRN identifies its unique features and provides a basis for structure-guided design of inhibitors.
A double role of the Gal80 N terminus in activation of transcription by Gal4pActivation of gene expression by Gal4p in K. lactis requires an element in the N terminus of KlGal80p that mediates nuclear co-import of KlGal1p and galactokinase inhibition to support the co-inducer function of KlGal1p.
Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeastThe canonical LINC complex is composed of two different transmembrane proteins; this work reveals the heterotrimeric composition of the telomere-associated LINC complex in budding yeast.
Implications for tetraspanin-enriched microdomain assembly based on structures of CD9 with EWI-FCrystal and single-particle cryo-EM structures reveal how the tetraspanin CD9 interacts with its prototypical partner EWI-F and provide a new concatenation model for the assembly of tetraspanin-enriched microdomains.
Stoichiometry of Rtt109 complexes with Vps75 and histones H3-H4The work determines the relative and absolute stoichiometry of a 5-chain protein complex involved in histone chaperoning and acetylation. Using sedimentation velocity and light scattering, it uncovers a dynamic equilibrium of complex self-association.