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Structural Biology

  • Structural studies on ribosomes of differentially macrolide-resistant <em>Staphylococcus aureus</em> strains
    Open Access
    Structural studies on ribosomes of differentially macrolide-resistant Staphylococcus aureus strains

    André Rivalta, Aliza Fedorenko, Alexandre Le Scornet, Sophie Thompson, Yehuda Halfon, Elinor Breiner Goldstein, Sude Çavdaroglu, Tal Melenitzky, Disha-Gajanan Hiregange, Ella Zimmerman, Anat Bashan, Mee-Ngan Frances Yap, Ada Yonath

    André Rivalta ... Ada Yonath

    Published 9 June 2025

    Macrolide resistance in Staphylococcus aureus is mediated by Erm-family methyltransferases, altering ribosomal drug binding. Using cryo-EM, we analyze solithromycin-bound ribosomes from resistant strains, revealing interactions that may enhance antibiotic efficacy.

  • The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro
    Open Access
    The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro

    Xiangyi S Wang, Jenny Jiou, Anthony Cerra, Simon A Cobbold, Marco Jochem, Ka Hin Toby Mak, Leo Corcilius, John Silke, Richard J Payne, Ethan D Goddard-Borger, David Komander, Bernhard C Lechtenberg

    Xiangyi S Wang ... Bernhard C Lechtenberg

    Published 1 April 2025

    The RBR E3 ligase HOIL-1 ubiquitinates Ser/Thr residues and various disaccharides in vitro and can be used to generate ubiquitinated sugars as tools to study non-proteinaceous ubiquitination.

  • Molecular mechanism of bacteriophage contraction structure of an S-layer–penetrating bacteriophage
    Open Access
    Molecular mechanism of bacteriophage contraction structure of an S-layer–penetrating bacteriophage

    Jason S Wilson, Louis-Charles Fortier, Robert P Fagan, Per A Bullough

    Jason S Wilson ... Per A Bullough

    Published 26 March 2025

    This study describes the high-resolution cryoEM structure of a contractile bacteriophage, displaying reduced contraction and lack of enzymatic domains for penetration.

  • Mechanical forces and ligand binding modulate <em>Pseudomonas aeruginosa</em> PilY1 mechanosensitive protein
    Open Access
    Mechanical forces and ligand binding modulate Pseudomonas aeruginosa PilY1 mechanosensitive protein

    Francisco J Cao-Garcia, Jane E Walker, Stephanie Board, Alvaro Alonso-Caballero

    Francisco J Cao-Garcia ... Alvaro Alonso-Caballero

    Published 7 March 2025

    Single-molecule experiments reveal that PilY1’s behavior changes with force load and ligand binding, allowing for the mechanical compartmentalization of its functions.

  • MDC1 mediates Pellino recruitment to sites of DNA double-strand breaks
    Open Access
    MDC1 mediates Pellino recruitment to sites of DNA double-strand breaks

    Mònica Torres Esteban, Matthew J Stewart, Eilis Bragginton, Alice Meroni, Annica Pellizzari, Alain Jeanrenaud, Stephen J Smerdon, Manuel Stucki

    Mònica Torres Esteban ... Manuel Stucki

    Published 6 March 2025

    RING E3 ubiquitin ligases Pellino 1 and 2 are recruited to sites of DNA damage via their non-canonical FHA domains that recognise conserved phosphorylated sequence motifs in the DNA damage adaptor protein MDC1.

  • Cryo-EM structures reveal the H<sup>+</sup>/citrate symport mechanism of <em>Drosophila</em> INDY
    Open Access
    Cryo-EM structures reveal the H+/citrate symport mechanism of Drosophila INDY

    Subin Kim, Jun Gyou Park, Seung Hun Choi, Ji Won Kim, Mi Sun Jin

    Subin Kim ... Mi Sun Jin

    Published 30 January 2025

    We provide cryo-EM structures of Drosophila INDY in diverse functional states at near-atomic resolution. Our structures reveal its conformational cycle, sodium-independent H+/citrate co-transport, and phospholipid effects on stability. These findings provide molecular insights into the enigmatic transport mechanism of INDY.

  • 4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hosts
    Open Access
    4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hosts

    Michael Taschner, Joe Bradley Dickinson, Florian Roisné-Hamelin, Stephan Gruber

    Michael Taschner ... Stephan Gruber

    Published 2 December 2024

    To streamline expression construct preparation for screening in diverse expression hosts, we present vectors and protocols using Golden Gate ligation to guide one-step Gibson assembly.

  • Structural roles of Ump1 and β-subunit propeptides in proteasome biogenesis
    Open Access
    Structural roles of Ump1 and β-subunit propeptides in proteasome biogenesis

    Eric Mark, Paula C Ramos, Fleur Kayser, Jörg Höckendorff, R Jürgen Dohmen, Petra Wendler

    Eric Mark ... Petra Wendler

    Published 11 September 2024

    A 2.1 Å cryo-EM structure of a late proteasome precursor reveals roles of the assembly chaperone Ump1 and β-subunit propeptides in proteasome biogenesis.

  • Cryo-EM reveals a phosphorylated R-domain envelops the NBD1 catalytic domain in an ABC transporter
    Open Access
    Cryo-EM reveals a phosphorylated R-domain envelops the NBD1 catalytic domain in an ABC transporter

    Rodolpho Souza Amado de Carvalho, Md Shamiul Islam Rasel, Nitesh K Khandelwal, Thomas M Tomasiak

    Rodolpho Souza Amado de Carvalho ... Thomas M Tomasiak

    Published 29 August 2024

    Cryo-EM reveals that the R-domain of an ABCC transporter encircles the ATP catalytic domain-1 in a tetra-phosphorylated manner and regulates the ATPase activity along with cellular transport.

  • Activation mechanism and novel binding sites of the BK<sub>Ca</sub> channel activator CTIBD
    Open Access
    Activation mechanism and novel binding sites of the BKCa channel activator CTIBD

    Narasaem Lee, Subin Kim, Na Young Lee, Heeji Jo, Pyeonghwa Jeong, Haushabhau S Pagire, Suvarna H Pagire, Jin Hee Ahn, Mi Sun Jin, Chul-Seung Park

    Narasaem Lee ... Chul-Seung Park

    Published 1 August 2024

    CTIBD relieves overactive bladder in animal models by activating BKCa channels through extracellular binding, independent of calcium and membrane depolarization; key residues include W22, W203, and F266.

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