Stem Cells
Robust production of uniform human cerebral organoids from pluripotent stem cellsBy leveraging advances in 3D stem cell culture techniques, the authors describe, characterize and validate a novel platform to efficiently generate morphologically consistent human forebrain cerebral organoids.
The molecular clock protein Bmal1 regulates cell differentiation in mouse embryonic stem cellsAnalysis of the role of the molecular clock protein Bmal1 in pluripotent embryonic stem cells reveals a novel function of circadian proteins in cell differentiation and early embryo development.
BMAL1 coordinates energy metabolism and differentiation of pluripotent stem cellsAlthough pluripotent cells lack circadian oscillations, this study reveals a novel function of the master circadian regulator BMAL1 in metabolism and early differentiation of embryonic stem cells.
The stress polarity signaling (SPS) pathway serves as a marker and a target in the leaky gut barrier: implications in aging and cancerUsing patient-derived organoids the authors show how a specialized polarity pathway protects our gut barrier from stress-induced collapse. Findings highlight both diagnostic and therapeutic potential of the pathway for treating gut barrier dysfunction in aging, cancer, and dysbiosis.
Metabolic reprogramming of fibro/adipogenic progenitors facilitates muscle regenerationHigh-fat diet ameliorates muscle dystrophic phenotype by promoting the FAP-dependent myogenesis of satellite cells.
Refining the adipose progenitor cell landscape in healthy and obese visceral adipose tissue using single-cell gene expression profilingThis work dissects adipose progenitor cell (APC) heterogeneity in normal and obese adipose tissue using single-cell expression profiling. Novel APC subpopulations are identified and characterized.
Defining the expression of piRNA and transposable elements in Drosophila ovarian germline stem cells and somatic support cellsComprehensive transcriptional characterization of mRNA and small RNA in early Drosophila germline stem cells reveals novel piRNA clusters, transposon dynamics, and alternative splicing events.
Endogenous epitope-tagging of Tet1, Tet2 and Tet3 identifies TET2 as a naïve pluripotency markerExpression of TET proteins in pluripotent cell types is visualised by epitope tagging of endogenous alleles.
Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modificationsThe glycine cleavage system (GCS) is highly activated to promote stem cell pluripotency. The GCS catabolizes glycine to prevent methylglyoxal accumulation and to fuel H3K4me3 modification, promoting the expression of pluripotency genes.
Constitutive STAT5 activation regulates Paneth and Paneth-like cells to control Clostridium difficile colitisSTAT5 controls the intestinal epithelial stem cell niche by regulating Wnt/β-catenin signaling, and lack of active pYSTAT5 induces Clostridium difficile infection. STAT5 may be a transcription factor that restricts niche cell differentiation.