Stem Cells
- Refining the adipose progenitor cell landscape in healthy and obese visceral adipose tissue using single-cell gene expression profiling
This work dissects adipose progenitor cell (APC) heterogeneity in normal and obese adipose tissue using single-cell expression profiling. Novel APC subpopulations are identified and characterized.
- Defining the expression of piRNA and transposable elements in Drosophila ovarian germline stem cells and somatic support cells
Comprehensive transcriptional characterization of mRNA and small RNA in early Drosophila germline stem cells reveals novel piRNA clusters, transposon dynamics, and alternative splicing events.
- Endogenous epitope-tagging of Tet1, Tet2 and Tet3 identifies TET2 as a naïve pluripotency marker
Expression of TET proteins in pluripotent cell types is visualised by epitope tagging of endogenous alleles.
- Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications
The glycine cleavage system (GCS) is highly activated to promote stem cell pluripotency. The GCS catabolizes glycine to prevent methylglyoxal accumulation and to fuel H3K4me3 modification, promoting the expression of pluripotency genes.
- Constitutive STAT5 activation regulates Paneth and Paneth-like cells to control Clostridium difficile colitis
STAT5 controls the intestinal epithelial stem cell niche by regulating Wnt/β-catenin signaling, and lack of active pYSTAT5 induces Clostridium difficile infection. STAT5 may be a transcription factor that restricts niche cell differentiation.
- ROS amplification drives mouse spermatogonial stem cell self-renewal
Although reactive oxygen species (ROS) are required for spermatogonial stem cell (SSC) self-renewal, the mechanism has remained unknown. We show that SSC self-renewal signals activate MAPK14/MAPK7 pathway to induce nuclear translocation of BCL6B and activation of NOX1.
- Location-dependent maintenance of intrinsic susceptibility to mTORC1-driven tumorigenesis
Per-cell quantification of mTORC1 signaling activity in neural stem/progenitor cells reveals differential signaling, proliferative, and tumor-forming capability between dorsal and ventral cells within a single niche.
- Assessment and site-specific manipulation of DNA (hydroxy-)methylation during mouse corticogenesis
This work describes the dynamics of DNA modifications in specific cell types of the developing mammalian cortex. By providing a new method to manipulate this process in vivo, it is shown how this process can influence brain formation.
- Polycomb and Notch signaling regulate cell proliferation potential during Caenorhabditis elegans life cycle
Caenorhabditis elegans lineage is invariant between animals. By challenging cell fate in differentiated worms, an unexpected role of Polycomb and Notch signaling in the control of cell proliferation was uncovered, suggesting that the lineage is more flexible than believed.
- A C/EBPα–Wnt connection in gut homeostasis and carcinogenesis
This research reveals an antagonism between C/EBPα expression and activated Wnt signaling in the human and mouse gut and suggests a tumor suppressor function of C/EBPα in human and murine intestinal cancer.