Stem Cells
Harnessing reaction-based probes to preferentially target pancreatic β-cells and β-like cellsThe study uses a reaction probe to target insulin-expressing pancreatic β-cells and β-like cells derived from pluripotent stem cells by harnessing high intracellular Zn(II) concentration.
The Paf1 complex positively regulates enhancer activity in mouse embryonic stem cellsUsing ChIP-seq and functional genomic analyses, the study shows that the Paf1 complex occupies transcriptional enhancers and positively regulates their activity.
Scratch-induced partial skin wounds re-epithelialize by sheets of independently migrating keratinocytesIntravital microscopy of scratch wounds in the murine skin reveals that individual basal keratinocytes migrate in a swarming fashion towards the wound to bypass intact hair follicles, thereby facilitating fast repair.
Myeloid transformation by MLL-ENL depends strictly on C/EBPThis publication shows that C/EBP (CCAAT enhancer–binding protein) transcription factors are mandatory for maintaining the myeloid leukemic phenotype, suggesting a therapeutic option by interfering with the non-oncogenic C/EBP dependence.
MicroRNA regulation of murine trophoblast stem cell self-renewal and differentiationPost-transcriptional regulation of CDX2 and cell cycle genes by miR-290 and miR-322 clusters along with transactivation of miR-290 cluster and cyclin D1 by CDX2 equipoise trophoblast stem cell self-renewal and differentiation.
Genetic targeting of neurogenic precursors in the adult forebrain ventricular epitheliumIn vivo evidence for precursors that produce neurons independent of neurosphere-forming neural stem cells suggests the adult forebrain, like the developing brain, has two distinct neurogenic pathways.
Robust production of uniform human cerebral organoids from pluripotent stem cellsBy leveraging advances in 3D stem cell culture techniques, the authors describe, characterize and validate a novel platform to efficiently generate morphologically consistent human forebrain cerebral organoids.
The molecular clock protein Bmal1 regulates cell differentiation in mouse embryonic stem cellsAnalysis of the role of the molecular clock protein Bmal1 in pluripotent embryonic stem cells reveals a novel function of circadian proteins in cell differentiation and early embryo development.
BMAL1 coordinates energy metabolism and differentiation of pluripotent stem cellsAlthough pluripotent cells lack circadian oscillations, this study reveals a novel function of the master circadian regulator BMAL1 in metabolism and early differentiation of embryonic stem cells.
The stress polarity signaling (SPS) pathway serves as a marker and a target in the leaky gut barrier: implications in aging and cancerUsing patient-derived organoids the authors show how a specialized polarity pathway protects our gut barrier from stress-induced collapse. Findings highlight both diagnostic and therapeutic potential of the pathway for treating gut barrier dysfunction in aging, cancer, and dysbiosis.