Stem Cells
Differential modulation of polycomb-associated histone marks by cBAF, pBAF, and gBAF complexesThis study shows that the canonical BAF (cBAF) complex uniquely opposes polycomb-associated repressive marks at the Nkx2-9 gene in mouse embryonic stem cells, leading to transcriptional activation, a function not shared by gBAF or pBAF complexes.
Helicobacter pylori induces the expression of Lgr5 and stem cell properties in gastric target cellsHelicobacter pylori infection induces Lgr5 expression and stem cell properties in its target cells in a type IV secretion system/NF-kB-dependent manner. This effect on stemness is exacerbated by constitutive Wnt pathway activation and persists after antibiotic eradication.
ER procollagen storage defect without coupled unfolded protein response drives precocious arthritisThe molecular basis of a collagenopathy is evaluated in an expandable human cartilage model of disease. The pathologic Gly1170Ser substitution causes an ER storage phenotype without inducing the unfolded protein response. This cartilage model could accelerate screening for potential therapies.
STAT3 inhibition recovers regeneration of aged muscles by restoring autophagy in muscle stem cellsThis study shows that STAT3 regulates MuSC function by both transcription-dependent and transcription-independent control of autophagy. STAT3 inhibition reverses age-dependent impairment of muscle regeneration, by restoring autophagy in aged MuSCs.
GDF15 controls primary cilia morphology and function thereby affecting progenitor proliferationGDF15/GFRAL ciliary signalling affects cilia morphology, function and proliferation of progenitors contacting the lateral ventricle from late development onwards.
Lineage-specific changes in mitochondrial properties during neural stem cell differentiationDuring SVZ-derived NSC differentiation, Mfn1/2 levels increase, whereas DRP1 levels significantly decrease. Alterations in mitochondrial morphology and bioenergetics are lineage-specific.
Generation of marmoset primordial germ cell–like cells under chemically defined conditionsMarmoset primordial germ cells (PGCs) express SOX17, AP2Ɣ, and BLIMP1 in vivo, and under specific feeder-free conditions, marmoset iPSCs can be induced into PGC-like cells in vitro.
Oxidative phosphorylation is a pivotal therapeutic target of fibrodysplasia ossificans progressivaOxidative phosphorylation plays a pivotal role in heterotopic ossification associated with fibrodysplasia ossificans progressiva, underscoring its potential as a significant therapeutic target.
Reduced myeloid commitment and increased uptake by macrophages of stem cell–derived HPS2 neutrophilsOur current work uses iPSC-derived neutrophils to unveil novel reasons for the neutropenia found in HPS2 patients. HPS2 iPSC-derived neutrophils have lower myeloid commitment, and are actively phagocytosed by macrophages present in the iPSC cultures or healthy donor macrophages.
Chromatin targeting of the RNF12/RLIM E3 ubiquitin ligase controls transcriptional responsesThe E3 ubiquitin ligase RNF12/RLIM is mutated in intellectual disability and controls developmental gene expression. We show that chromatin targeting of RNF12 is required for substrate ubiquitylation and transcriptional responses, providing insight into mechanisms by which ubiquitylation regulates gene expression.