Stem Cells
- Generation of marmoset primordial germ cell–like cells under chemically defined conditions
Marmoset primordial germ cells (PGCs) express SOX17, AP2Ɣ, and BLIMP1 in vivo, and under specific feeder-free conditions, marmoset iPSCs can be induced into PGC-like cells in vitro.
- Oxidative phosphorylation is a pivotal therapeutic target of fibrodysplasia ossificans progressiva
Oxidative phosphorylation plays a pivotal role in heterotopic ossification associated with fibrodysplasia ossificans progressiva, underscoring its potential as a significant therapeutic target.
- Reduced myeloid commitment and increased uptake by macrophages of stem cell–derived HPS2 neutrophils
Our current work uses iPSC-derived neutrophils to unveil novel reasons for the neutropenia found in HPS2 patients. HPS2 iPSC-derived neutrophils have lower myeloid commitment, and are actively phagocytosed by macrophages present in the iPSC cultures or healthy donor macrophages.
- Chromatin targeting of the RNF12/RLIM E3 ubiquitin ligase controls transcriptional responses
The E3 ubiquitin ligase RNF12/RLIM is mutated in intellectual disability and controls developmental gene expression. We show that chromatin targeting of RNF12 is required for substrate ubiquitylation and transcriptional responses, providing insight into mechanisms by which ubiquitylation regulates gene expression.
- Early Atf4 activity drives airway club and goblet cell differentiation
PERK/Atf4 drives airway epithelial differentiation, impacting secretory cell numbers, revealing ATF4’s role in respiratory tract regeneration.
- Single-cell time series analysis reveals the dynamics of HSPC response to inflammation
Unbiased single-cell time series analysis of gene expression response to inflammation, using novel computational approaches, links myeloid depletion to alterations in differentiation bias and pyroptosis activity.
- PD-L1 regulates inflammatory programs of macrophages from human pluripotent stem cells
This study identified the novel role of immune checkpoint PD-L1 in inflammatory programs of macrophages.
- Incomplete activation of Alyref and Gabpb1 leads to preimplantation arrest in cloned mouse embryos
This study shows that failed activation of developmentally required Alyref and Gabpb1 genes leads to the arrest of cloned mouse embryos.
- Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generation
This article describes a new method to evaluate the actomyosin activity in living cardiomyocytes during beating based on nonlinear scattering microscopy, which will be useful for elucidating cardiomyopathy mechanisms, efficacy of an intervention, and others.
- Modeling fibrotic alveolar transitional cells with pluripotent stem cell-derived alveolar organoids
In vitro modeling of transitional alveolar epithelial cells in idiopathic pulmonary fibrosis by the use of a fibrosis cocktail and alveolar organoids from induced pluripotent stem cells.