Stem Cells
PD-L1 regulates inflammatory programs of macrophages from human pluripotent stem cellsThis study identified the novel role of immune checkpoint PD-L1 in inflammatory programs of macrophages.
Incomplete activation of Alyref and Gabpb1 leads to preimplantation arrest in cloned mouse embryosThis study shows that failed activation of developmentally required Alyref and Gabpb1 genes leads to the arrest of cloned mouse embryos.
Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generationThis article describes a new method to evaluate the actomyosin activity in living cardiomyocytes during beating based on nonlinear scattering microscopy, which will be useful for elucidating cardiomyopathy mechanisms, efficacy of an intervention, and others.
Modeling fibrotic alveolar transitional cells with pluripotent stem cell-derived alveolar organoidsIn vitro modeling of transitional alveolar epithelial cells in idiopathic pulmonary fibrosis by the use of a fibrosis cocktail and alveolar organoids from induced pluripotent stem cells.
Origin and segregation of the human germlineHuman primordial germ cells originate in peri-gastrulation embryos from the posterior epiblast precursors with an obligatory role for TFAP2A, which is redundant for contribution to the amnion.
Evaluation of genetic demultiplexing of single-cell sequencing data from model speciesThe authors demonstrate that scRNA-seq sample pooling followed by genetics-based separation of individuals is an effective means to identify individual samples in a variety of commonly studied species.
Validating organoid-derived human intestinal monolayers for personalized therapy in cystic fibrosisUssing chamber studies of intestinal organoid monolayers compare favorably to established preclinical tools to assess individual responses to CFTR modulator drugs in people with cystic fibrosis.
SMN promotes mitochondrial metabolic maturation during myogenesis by regulating the MYOD-miRNA axisSMN contributes to muscle maturation by binding to the promoter regions of MYOD1 and miRNAs and regulating the expression of these molecules during myogenesis.
Trans-differentiation of trophoblast stem cells: implications in placental biologyFormation of trophendothelial cells is associated with trophoblast differentiation and is regulated by HES1. Trophoblast differentiation–induced TRAIL secretion directs preferential demise of ECs located in their vicinity, aiding trophoblast vasculogenic mimicry.
The mechanosensitive ion channel PIEZO1 promotes satellite cell function in muscle regenerationPIEZO1, a mechanosensitive ion channel, regulates Rho-GTPase activity and specifically accumulates at the midbody of muscle satellite cells to precisely control cell division for muscle regeneration.