Stem Cells
Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26This study provides molecular insights into RNA target recognition by the TRIM-NHL protein Mei-P26 that functions in Drosophila ovarian germline stem cell maintenance, oogenesis, and spermatogenesis.
Reduced adhesion of aged intestinal stem cells contributes to an accelerated clonal driftAnalysis of clonal dynamics of intestinal stem cells supports an accelerated clonal drift upon aging, likely because of reduced adhesion of aged ISCs because of reduced canonical Wnt signaling.
Nutrient sensitive protein O-GlcNAcylation modulates the transcriptome through epigenetic mechanisms during embryonic neurogenesisMaternal hyperglycemia and elevated O-GlcNAc levels perturb promoter bivalency and lead to transcriptional up-regulation of neurogenic transcription factors during embryonic neurogenesis.
Single-cell RNA-seq reveals heterogeneity in hiPSC-derived muscle progenitors and E2F family as a key regulator of proliferationThis study identified and characterized four different populations of muscle progenitor cells derived from human induced pluripotent stem cells.
AGO1 regulates pericentromeric regions in mouse embryonic stem cellsDepletion of AGO1 in mESCs leads to a redistribution of H3K9me3 and HP1α away from pericentromeric regions and is accompanied by an up-regulation of major satellites transcripts.
Muscle stem cell polarity requires QKI-mediated alternative splicing of Integrin Alpha-7 (Itga7)The RNA-binding protein Quaking (QKI) is a post-transcriptional regulator of genes encoding polarity proteins in muscle stem cells. Loss of QKI in MuSCs results in reduced myogenic progenitors and a striking muscle regeneration defect.
HERVH-derived lncRNAs negatively regulate chromatin targeting and remodeling mediated by CHD7Mutations in CHD7 are diagnostic for human CHARGE syndrome. RNAs expressed from the HERVH repeats modulate CHD7 function providing one mechanism for regulation of differentiation of pluripotent cells.
Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlineA TPP1 mutation known to cause telomere shortening and bone marrow failure in humans recapitulates telomere loss but results in severe germline defects in mice without impacting murine hematopoiesis.
Kinomics platform using GBM tissue identifies BTK as being associated with higher patient survivalBTK is a dominant bioactive kinase expressed within both cancer and immune cells of GBM tissue. Complex cell co-cultures might better model the impact of kinase inhibitors as therapeutics in GBM.
Loss of Resf1 reduces the efficiency of embryonic stem cell self-renewal and germline entryRESF1 supports ESC self-renewal by raising expression of transmembrane LIF receptor and key pluripotency transcription factors and increases in vitro primordial germ cell differentiation efficiency.