Molecular Diagnostics & Diagnostic Imaging
Sensitive visualization of SARS-CoV-2 RNA with CoronaFISHWe design an RNA-FISH method to target the plus and minus strands of SARS-CoV-2 RNA, and apply it to image the virus in cell lines, lung tissue, and nasal swabs, by fluorescence and electron microscopy.
Engineered RNA biosensors enable ultrasensitive SARS-CoV-2 detection in a simple color and luminescence assayThis work reports engineered toehold RNA–based biosensors for COVID-19 diagnostics, with a simple color or luminescence readout that makes it easily deployable in both well-equipped labs as well as low resource settings.
A serum proteome signature to predict mortality in severe COVID-19 patientsA serum proteome panel, which includes several type-3 cystatins and inter-α-trypsin inhibitors, predicts mortality in severe COVID-19 patients very well, in three independent cohorts.
Prognostic accuracy of MALDI-TOF mass spectrometric analysis of plasma in COVID-19High mortality rate in SARS-CoV-2–infected individuals requires accurate markers for predicting COVID-19 severity. MALDI-TOF analysis revealed differential plasma levels of SAA1 and SAA2 associated with higher risk of hospitalization and can be used to improve COVID-19 monitoring and therapy.
Comparative genomics of Glandirana rugosa using unsupervised AI reveals a high CG frequencyGenome sequencing of a unique frog (Glandirana rugosa) having XY/ZW systems within the species and comparative genomics with other six frogs were performed using a batch-learning self-organizing map, which is unsupervised AI for oligonucleotide compositions, to clarify its genome characteristics.
Ubiquitin and SUMO conjugation as biomarkers of acute myeloid leukemias response to chemotherapiesThis study describes the identification of a new class of biomarkers of cancer response to therapies based on protein modification by ubiquitin and SUMO and developed tools to validate them in acute myeloid leukemia patient samples.
Reference-free transcriptome exploration reveals novel RNAs for prostate cancer diagnosisA reference-free computational workflow for the discovery of unannotated RNA biomarkers retrieves novel noncoding RNAs with high potential for prostate cancer diagnosis.