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Molecular Biology

  • Silica-based solid-phase extraction of cross-linked nucleic acid–bound proteins
    Open Access
    Silica-based solid-phase extraction of cross-linked nucleic acid–bound proteins

    Claudio Asencio, Aindrila Chatterjee, Matthias W Hentze

    Claudio Asencio ... Matthias W Hentze

    Published 18 June 2018

    The 2C method allows the rapid and straightforward isolation of nucleic acid–protein complexes, greatly simplifying downstream applications for the study of DNA– and RNA–protein interactions.

  • DHX15 regulates CMTR1-dependent gene expression and cell proliferation
    Open Access
    DHX15 regulates CMTR1-dependent gene expression and cell proliferation

    Francisco Inesta-Vaquera, Viduth K Chaugule, Alison Galloway, Laurel Chandler, Alejandro Rojas-Fernandez, Simone Weidlich, Mark Peggie, Victoria H Cowling

    Francisco Inesta-Vaquera ... Victoria H Cowling

    Published 18 June 2018

    DHX15 helicase regulates CMTR1-dependent first transcribed nucleotide ribose O-2 methylation.

  • Co-existing feedback loops generate tissue-specific circadian rhythms
    Open Access
    Co-existing feedback loops generate tissue-specific circadian rhythms

    J Patrick Pett, Matthew Kondoff, Grigory Bordyugov, Achim Kramer, Hanspeter Herzel

    J Patrick Pett ... Hanspeter Herzel

    Published 14 June 2018

    The analysis of tissue-specific data-based models of the gene regulatory network of the mammalian circadian clock reveals organ-specific synergies of feedback loops.

  • Ribosome recycling is coordinated by processive events in two asymmetric ATP sites of ABCE1
    Open Access
    Ribosome recycling is coordinated by processive events in two asymmetric ATP sites of ABCE1

    Elina Nürenberg-Goloub, Holger Heinemann, Milan Gerovac, Robert Tampé

    Elina Nürenberg-Goloub ... Robert Tampé

    Published 14 June 2018

    The stepwise ribosome disassembly in the translation cycle of eukaryotes and archaea is scheduled by discrete molecular events within the asymmetric ribosome recycling factor ABCE1.

  • ERα activity depends on interaction and target site corecruitment with phosphorylated CREB1
    Open Access
    ERα activity depends on interaction and target site corecruitment with phosphorylated CREB1

    Melissa Berto, Valerie Jean, Wilbert Zwart, Didier Picard

    Melissa Berto ... Didier Picard

    Published 7 June 2018

    CREB1 interacts with, stimulates, and is necessary for estrogen receptor α activation by estrogen or cAMP signaling, and this cooperation may underlie their anti-apoptotic activities in breast cancer cells.

  • Regulation of microtubule dynamic instability by the carboxy-terminal tail of β-tubulin
    Open Access
    Regulation of microtubule dynamic instability by the carboxy-terminal tail of β-tubulin

    Colby P Fees, Jeffrey K Moore

    Colby P Fees ... Jeffrey K Moore

    Published 19 April 2018

    This work examines how the carboxy-terminal tail domain of β-tubulin governs microtubule dynamic instability and the structure of plus ends using complementary in vivo and in vitro experiments.

  • CHRAC/ACF contribute to the repressive ground state of chromatin
    Open Access
    CHRAC/ACF contribute to the repressive ground state of chromatin

    Alessandro Scacchetti, Laura Brueckner, Dhawal Jain, Tamas Schauer, Xu Zhang, Frank Schnorrer, Bas van Steensel, Tobias Straub, Peter B Becker

    Alessandro Scacchetti ... Peter B Becker

    Published 9 February 2018

    Chromatin accessibility complex/ATP-utilizing chromatin assembly and remodeling factor help to establish basal transcriptional repression, conceivably through improving the regular spacing of nucleosomes in euchromatin.

  • The ER morphology-regulating lunapark protein induces the formation of stacked bilayer discs
    Open Access
    The ER morphology-regulating lunapark protein induces the formation of stacked bilayer discs

    Songyu Wang, Robert E Powers, Vicki AM Gold, Tom A Rapoport

    Songyu Wang ... Tom A Rapoport

    Published 19 January 2018

    Lunapark, an ER protein implicated in stabilizing junctions of the tubular network and regulating morphology changes during mitosis, forms stacked bilayer discs upon reconstitution with phospholipids.

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