Molecular Biology
- Silica-based solid-phase extraction of cross-linked nucleic acid–bound proteins
The 2C method allows the rapid and straightforward isolation of nucleic acid–protein complexes, greatly simplifying downstream applications for the study of DNA– and RNA–protein interactions.
- DHX15 regulates CMTR1-dependent gene expression and cell proliferation
DHX15 helicase regulates CMTR1-dependent first transcribed nucleotide ribose O-2 methylation.
- Co-existing feedback loops generate tissue-specific circadian rhythms
The analysis of tissue-specific data-based models of the gene regulatory network of the mammalian circadian clock reveals organ-specific synergies of feedback loops.
- Ribosome recycling is coordinated by processive events in two asymmetric ATP sites of ABCE1
The stepwise ribosome disassembly in the translation cycle of eukaryotes and archaea is scheduled by discrete molecular events within the asymmetric ribosome recycling factor ABCE1.
- ERα activity depends on interaction and target site corecruitment with phosphorylated CREB1
CREB1 interacts with, stimulates, and is necessary for estrogen receptor α activation by estrogen or cAMP signaling, and this cooperation may underlie their anti-apoptotic activities in breast cancer cells.
- Regulation of microtubule dynamic instability by the carboxy-terminal tail of β-tubulin
This work examines how the carboxy-terminal tail domain of β-tubulin governs microtubule dynamic instability and the structure of plus ends using complementary in vivo and in vitro experiments.
- CHRAC/ACF contribute to the repressive ground state of chromatin
Chromatin accessibility complex/ATP-utilizing chromatin assembly and remodeling factor help to establish basal transcriptional repression, conceivably through improving the regular spacing of nucleosomes in euchromatin.
- The ER morphology-regulating lunapark protein induces the formation of stacked bilayer discs
Lunapark, an ER protein implicated in stabilizing junctions of the tubular network and regulating morphology changes during mitosis, forms stacked bilayer discs upon reconstitution with phospholipids.