Molecular Biology
- Drosophila pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenes
Drosophila pVALIUM10 TRiP RNAi lines cause undesirable knockdown of transgenic reporters and overexpression lines generated with the help of the Gateway cloning technology by targeting attB sites.
- Transcriptome analyses in infertile men reveal germ cell–specific expression and splicing patterns
We uncover extensive germ cell type–dependent transcriptome changes in human spermatogenesis and demonstrate the potential of alternative splicing in the regulation of spermatogenesis.
- Hepatic DKK1-driven steatosis is CD36 dependent
Increased DKK1 in hepatic steatosis contributes CD36-mediated fatty acid uptake and insulin resistance.
- Discovery of RUF6 ncRNA–interacting proteins involved in P. falciparum immune evasion
An RNA-directed proteomic discovery (ChIRP-MS) protocol is used to identify in vivo RUF6 ncRNA–protein interactions that sustain virulence genes in the human malaria parasite, Plasmodium falciparum.
- Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents used
Mono(ADP-ribosyl)ation is an emerging modification, for which recently detection reagents were generated. We cross-validated all reagents and observe remarkable differences in efficiency, depending on the substrate investigated and on the methods that are used.
- In vivo probing of SECIS-dependent selenocysteine translation in Archaea
By turning a bacterial reporter into an archaeal selenoprotein, in vivo probing of structure–function relations during UGA recoding for selenoprotein synthesis in Archaea is greatly facilitated.
- Miro GTPase domains regulate the assembly of the mitochondrial motor–adaptor complex
Relocalizing the mitochondrial motor–adaptor protein Miro to peroxisomes and systematically manipulating each GTPase domain reveal the importance of the N-terminal GTPase domain of Miro1 for regulating mitochondrial transport.
- Palmitate impairs circadian transcriptomics in muscle cells through histone modification of enhancers
The disruption of circadian rhythms because of lipid overload may lead to epigenomic changes that influence metabolism. Thus, a dietary or therapeutic modulation of lipid levels, a cornerstone in the treatment of metabolic disorders, may prevent circadian misalignment in peripheral tissues.
- MDF is a conserved splicing factor and modulates cell division and stress response in Arabidopsis
MDF acts like its human homolog SART1 as a splicing factor that is important for maintenance of genome stability and correct splicing and expression of genes involved in cell division and stress response.
- Autoimmune RNA dysregulation and seizures: therapeutic prospects in neuropsychiatric lupus
Lupus autoantibodies directed at neuroregulatory BC200 RNA cause seizure susceptibility in mice, but sequestration with antigen prevents seizures, indicating utility in therapeutic interventions.