Molecular Biology
MBNL1 alternative splicing isoforms play opposing roles in cancerMBNL1 proteins lacking exon 7 (−ex7) are antisurvival factors with tumor suppressive role that cancer cells tend to down-regulate in favor of MBNL +ex7 isoforms.
Distinctive features of lincRNA gene expression suggest widespread RNA-independent functionsCombining single-cell RNA-seq of mouse ESCs and NPCs, lincRNA gene deletions, conditional RNA depletion, and nuclear exosome profiling distinguishes RNA-dependent and RNA-independent lincRNA gene activities.
Proteins of generalist and specialist pathogens differ in their amino acid compositionPrimitive amino acids and disorder content in secreted proteins are distinctively enriched in generalist compared with host-specific pathogens.
Role of Cnot6l in maternal mRNA turnoverMice lacking Cnot6l, a deadenylase component of the CCR4–NOT complex, are viable, but females have ∼40% smaller litters. Cnot6l is a maternal-effect gene acting in maternal mRNA degradation.
Dynamic m6A methylation facilitates mRNA triaging to stress granulesm6A modification in the 5′ vicinity of the coding sequence of transcripts provides a selective mechanism for triaging mRNAs to stress granules and is mediated by the YTHDF3 “reader” protein.
DNA polymerase ζ deficiency causes impaired wound healing and stress-induced skin pigmentationMice harboring DNA polymerase ζ–defective keratinocytes are shown to have a defect in wound healing and a striking p53-dependent migration of melanocytes to the skin following UV radiation or wounding.
Molecular analysis of human Ero1 reveals novel regulatory mechanisms for oxidative protein foldingThis study reveals novel regulatory mechanisms for human Ero1α and Ero1β, including cooperativity for oxygen binding and complex formation with PDI via a novel mixed disulfide linkage.
Somatic role of SYCE2: an insulator that dissociates HP1α from H3K9me3 and potentiates DNA repairThis study reveals that the synaptonemal complex protein SYCE2 potentiates ataxia telangiectasia mutated–mediated DNA double-strand break repair by dissociating HP1α from trimethylated histone H3 lysine 9 in somatic cells.
Recruitment of ubiquitin-activating enzyme UBA1 to DNA by poly(ADP-ribose) promotes ATR signallingHuman but not yeast UBA1 binds poly(ADP-ribose) polymers via a solvent-exposed and positively charged patch. Poly(ADP-ribose) polymerase 1–dependent recruitment of UBA1 to DNA ensures ataxia-telangiectasia and RAD3-related activation.
Chronic platelet-derived growth factor receptor signaling exerts control over initiation of protein translation in gliomaUsing phospho-proteomics in a new model of malignant glioma, we reveal that clinically relevant, chronic PDGFRα signaling differs considerably from acute receptor stimulation and unveils previously unrecognized control over key elements of the translation initiation machinery.