Molecular Biology
- Predictive data-driven modeling of C-terminal tyrosine function in the EGFR signaling network
The site-specific function of EGFR’s C-terminal tyrosines is interrogated by phosphotyrosine phosphoproteomics to build a data-driven model that links cell response to signaling nodes and elucidates novel associations in the EGFR signaling network.
- Structural insights into ubiquitin chain cleavage by Legionella ovarian tumor deubiquitinases
Legionella possesses OTU deubiquitinases (Lot DUBs), which hinder the host ubiquitin system. We performed structural and biochemical analyses on the Lot DUBs and explained how Lot DUBs are different from other OTU-DUBs.
- The cytochrome b carboxyl terminal region is necessary for mitochondrial complex III assembly
Synthesis/assembly of Cytb, encoded in the mitochondrial DNA, are regulated by the Cytb C-terminus. It modifies the interaction Cytb-Cbp3/Cbp6 to control Cytb and respiratory complex III biogenesis.
- New botulinum neurotoxin constructs for treatment of chronic pain
A new technique to make therapeutic botulinum neurotoxins via isopeptide bonding is described. The novel elongated botulinum neurotoxin can treat nerve injury pain without causing muscle paralysis.
- arfA antisense RNA regulates MscL excretory activity
Bacteria coordinate how they respond to translation and osmotic stress by using antisense RNA crosstalk between the genes involved in the respective stress response pathways.
- The role of Limch1 alternative splicing in skeletal muscle function
This works identifies a novel, alternatively spliced isoform of LIMCH1, that is, developmentally regulated and specific to skeletal muscle. Knocking out the six skeletal muscle–specific exons of LIMCH1 leads to impaired skeletal muscle function in vivo and ex vivo.
- Interaction hub critical for telomerase recruitment and primer-template handling for catalysis
By deleting, mutating, and adding back parts of the telomerase enzyme that extends chromosome ends, we uncover features important for its proper functioning in the cell.
- MicroRNAs in tear fluids predict underlying molecular changes associated with Alzheimer’s disease
This study demonstrated the translational potential of deregulated tear fluid miRNAs associated with amyloid beta production and proinflammation in a transgenic AD mouse model.
- Preference of CAMSAP3 for expanded microtubule lattice contributes to stabilization of the minus end
D2 region in CAMSAP3 preferentially bound to expanded microtubule lattices and also induced lattice expansion, explaining the molecular functions of CAMSAP3.
- The human MRS2 magnesium-binding domain is a regulatory feedback switch for channel activity
Magnesium (Mg2+) binding to the human mitochondrial RNA splicing 2 (MRS2) protein channel amino terminal domain disrupts homomeric interactions and inhibits mitochondrial Mg2+ uptake as a negative feedback mechanism.