Molecular Biology
- DCAF14 regulates CDT2 to promote SET8-dependent replication fork protection
DCAF14 modulates CRL4CDT2-dependent turnover during DNA replication. Loss of DCAF14 causes nascent strand degradation due to excessive proteasomal turnover of SET8 by aberrant CDT2 activity.
- Spt5 interacts genetically with Myc and is limiting for brain tumor growth in Drosophila
Knockdown of Spt5 in Drosophila affects various Myc-dependent phenotypes and delays the growth of a Myc-dependent brain tumor, thereby significantly extending the longevity of tumorous animals.
- Interconnections between m6A RNA modification, RNA structure, and protein–RNA complex assembly
This review summarizes current knowledge and future directions on the mechanisms that govern the interplay between m6A RNA modification, RNA folding, and protein–RNA interactions, focusing on the mechanisms and available quantitative information.
- Cell cycle-dependent gene networks for cell proliferation activated by nuclear CK2α complexes
Functional connection between activation of nuclear CK2α by phosphorylation which leads to association of CK2α with transcriptional start sites, and activation of histone genes, growth-related genes, and rDNA for cell proliferation.
- NRF2 connects Src tyrosine kinase to ferroptosis resistance in glioblastoma
The hyperactivation of Src tyrosine kinase in glioblastoma leads to the constitutive stabilization and activation of NRF2, thus causing resistance to ionizing radiation-induced ferroptosis.
- Identification of FAM53C as a cytosolic-anchoring inhibitory binding protein of the kinase DYRK1A
A previously uncharacterized protein, FAM53C, is identified as a binding partner for the kinase DYRK1A involved in numerous neurodevelopmental disorders, such as Down syndrome and autism spectrum disorder. FAM53C anchors the DYRK1A-DCAF7 complex within the cell cytoplasm in a kinase-inactive state.
- Improving lysosomal ferroptosis with NMN administration protects against heart failure
NMN supplementation affects lysosome-mediated ferroptosis, leading to improved autophagy, thereby reducing heart failure and prolonging the lifespan in mitochondrial translation-deficient mice.
- Functional plasticity in chromosome–microtubule coupling on the evolutionary time scale
We identify a lethal mutation in a subunit of the Dam1 complex, a chromosome–microtubule coupler in Saccharomyces cerevisiae with point centromeres. However, this mutation is tolerated in organisms with longer regional centromeres, possibly by employing more microtubules to capture each chromosome.
- Regulation of alternative splicing and polyadenylation in neurons
Neurons generate numerous cell-specific isoforms of genes that are expressed more broadly. Here, we review mechanisms that generate neural-specific alternative splicing and 3′UTRs and their biological impacts on the nervous system.
- TRIM25 targets p300 for degradation
Here, it is shown that the E3 protein TRIM25 targets the transcriptional co-activator p300 in an ubiquitin-independent manner for proteasomal degradation involving the motor protein dynein.