Molecular Biology
- The iron–sulfur helicase DDX11 promotes the generation of single-stranded DNA for CHK1 activation
The iron–sulfur cluster helicase DDX11 promotes the generation of ssDNA and the phosphorylation of CHK1 at serine-345, possibly by unwinding replication-dependent DNA secondary structures.
- Retrograde trafficking of Argonaute 2 acts as a rate-limiting step for de novo miRNP formation on endoplasmic reticulum–attached polysomes in mammalian cells
Intracellular trafficking of Argonaute 2 controls de novo miRNP formation on endoplasmic reticulum–attached polysomes in mammalian cells.
- In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joining
This study provides a new method that enables the labelling of knockout cells with fluorescent protein through microhomology-mediated end joining (MMEJ)–based knock-in.
- Dual role of a GTPase conformational switch for membrane fusion by mitofusin ubiquitylation
Mitochondrial fusion requires a conformational change in α4 of the GTPase domain, followed by AAA-ATPase regulation, thus revealing striking mechanistic similarities between small and large GTPases.
- Expression of AXL receptor tyrosine kinase relates to monocyte dysfunction and severity of cirrhosis
Immune dysfunction determines morbidity and mortality in liver cirrhosis. Distinct AXL-expressing circulating monocytes, which regulate antimicrobial responses, expand with progression of the disease.
- Refining the adipose progenitor cell landscape in healthy and obese visceral adipose tissue using single-cell gene expression profiling
This work dissects adipose progenitor cell (APC) heterogeneity in normal and obese adipose tissue using single-cell expression profiling. Novel APC subpopulations are identified and characterized.
- Plasticity in salt bridge allows fusion-competent ubiquitylation of mitofusins and Cdc48 recognition
Mitochondrial fusion requires an alternating salt bridge between CMT2A-associated disease residues that enable GTP hydrolysis; Fzo1 ubiquitylation then licenses post-fusion recycling by Cdc48.
- Nrf2 signaling links ER oxidative protein folding and calcium homeostasis in health and disease
Oxidative protein folding in the ER generates ROS, leading to Nrf2-dependent feedback on protein folding via ER calcium level modulation. This feedback loop is suppressed in ALS-associated mutant astrocytes but can be rescued by dimethyl fumarate.
- Conservation of cell-intrinsic immune responses in diverse nonhuman primate species
The transcriptomic response of diverse nonhuman primate (NHP) species to poly(I:C) is highly conserved, and this novel RNA sequencing dataset will help improve NHP genome annotations.
- Resolving kinesin stepping: one head at a time
This study shows for the first time a qualitative difference in the stepping of a heterodimeric motor and the asymmetric influence of autoinhibition on the stepping of one of the heads.