Molecular Biology
Chronic platelet-derived growth factor receptor signaling exerts control over initiation of protein translation in gliomaUsing phospho-proteomics in a new model of malignant glioma, we reveal that clinically relevant, chronic PDGFRα signaling differs considerably from acute receptor stimulation and unveils previously unrecognized control over key elements of the translation initiation machinery.
Silica-based solid-phase extraction of cross-linked nucleic acid–bound proteinsThe 2C method allows the rapid and straightforward isolation of nucleic acid–protein complexes, greatly simplifying downstream applications for the study of DNA– and RNA–protein interactions.
DHX15 regulates CMTR1-dependent gene expression and cell proliferationDHX15 helicase regulates CMTR1-dependent first transcribed nucleotide ribose O-2 methylation.
Co-existing feedback loops generate tissue-specific circadian rhythmsThe analysis of tissue-specific data-based models of the gene regulatory network of the mammalian circadian clock reveals organ-specific synergies of feedback loops.
Ribosome recycling is coordinated by processive events in two asymmetric ATP sites of ABCE1The stepwise ribosome disassembly in the translation cycle of eukaryotes and archaea is scheduled by discrete molecular events within the asymmetric ribosome recycling factor ABCE1.
ERα activity depends on interaction and target site corecruitment with phosphorylated CREB1CREB1 interacts with, stimulates, and is necessary for estrogen receptor α activation by estrogen or cAMP signaling, and this cooperation may underlie their anti-apoptotic activities in breast cancer cells.
Regulation of microtubule dynamic instability by the carboxy-terminal tail of β-tubulinThis work examines how the carboxy-terminal tail domain of β-tubulin governs microtubule dynamic instability and the structure of plus ends using complementary in vivo and in vitro experiments.
CHRAC/ACF contribute to the repressive ground state of chromatinChromatin accessibility complex/ATP-utilizing chromatin assembly and remodeling factor help to establish basal transcriptional repression, conceivably through improving the regular spacing of nucleosomes in euchromatin.
The ER morphology-regulating lunapark protein induces the formation of stacked bilayer discsLunapark, an ER protein implicated in stabilizing junctions of the tubular network and regulating morphology changes during mitosis, forms stacked bilayer discs upon reconstitution with phospholipids.