Molecular Biology
Non-redundant roles for the human mRNA decapping cofactor paralogs DCP1a and DCP1bThe study represents the first functional dissection of the two human cofactors present in the RNA decapping complex, and, significantly, it evaluates their role in the transcript buffering system.
Mast cell extracellular trap formation underlies vascular and neural injury and hyperalgesia in sickle cell diseaseMast cell extracellular traps catalyzed by peptidylarginine deiminase 4 contribute to vascular stasis and nerve injury, which may contribute to acute and chronic pain in sickle cell disease.
Regulation of proteostasis by sleep through autophagy in Drosophila models of Alzheimer’s diseaseSleep modulation alters Tau-induced neurodegeneration in Drosophila models of tauopathy.
Mitochondrial double-stranded RNA homeostasis depends on cell-cycle progressionThe accumulation of mitochondrial double-stranded RNA (mt-dsRNA) in cancer cells depends on cell proliferation status. Mt-dsRNA is more abundant in patient lung adenocarcinoma compared with healthy tissue.
PPM1G dephosphorylates eIF4E in control of mRNA translation and cell proliferationPPM1G inhibits cell proliferation by targeting phospho-eIF4E–dependent mRNA translation.
Activation mechanism and novel binding sites of the BKCa channel activator CTIBDCTIBD relieves overactive bladder in animal models by activating BKCa channels through extracellular binding, independent of calcium and membrane depolarization; key residues include W22, W203, and F266.
Late-life dietary folate restriction reduces biosynthesis without compromising healthspan in miceLow folate levels reduce anabolism, make yeast and worms live longer, and improve the metabolic flexibility of old mice.
Substrate diversity of NSUN enzymes and links of 5-methylcytosine to mRNA translation and turnoverThis study emphasises the emerging diversity of both, m5C writers and readers, affecting mRNA function and provides multiple new leads for future epitranscriptomic research.
E3 ligases RNF43 and ZNRF3 display differential specificity for endocytosis of Frizzled receptorsTransmembrane E3 ligases RNF43 and ZNRF3 selectively target specific Frizzled receptors to suppress WNT signalling, offering an explanation for the cancer tissue-specific distribution of RNF43 and ZNRF3 mutations.
Structure of the human systemic RNAi defective transmembrane protein 1 (hSIDT1) reveals the conformational flexibility of its lipid binding domainThis study reports a cryo-EM structure of a small non-coding RNA transporter, hSIDT1, and delineates the role of lipid in the conformational dynamics of the lipid binding domain of hSIDT1.