Molecular Biology
MAP4K3 inhibits Sirtuin-1 to repress the LKB1–AMPK pathway to promote amino acid-dependent activation of the mTORC1 complexOur results reveal the existence of a novel signaling pathway linking amino acid satiety with MAP4K3-dependent suppression of SIRT1 to inactivate the repressive LKB1–AMPK pathway and thereby potently activate the mTORC1 complex to dictate the metabolic disposition of the cell.
The CHARGE syndrome-associated protein FAM172A controls AGO2 nuclear importAGO2 has long been known to shuttle between the cytosol and the nucleus of mammalian cells and this study helps to understand how this occurs.
Prostaglandin F2α regulates mitochondrial dynamics and mitophagy in the bovine corpus luteumThis study investigates the early effects of PGF2α signaling on mitochondrial dynamics and mitophagy in bovine corpora lutea. Luteolytic mediator PGF2α, via PKC/ERK and AMPK signaling, activates mitochondrial fission and promotes PINK–Parkin mitophagy, placing mitochondria as novel targets in response to PGF2α.
Predictive data-driven modeling of C-terminal tyrosine function in the EGFR signaling networkThe site-specific function of EGFR’s C-terminal tyrosines is interrogated by phosphotyrosine phosphoproteomics to build a data-driven model that links cell response to signaling nodes and elucidates novel associations in the EGFR signaling network.
Structural insights into ubiquitin chain cleavage by Legionella ovarian tumor deubiquitinasesLegionella possesses OTU deubiquitinases (Lot DUBs), which hinder the host ubiquitin system. We performed structural and biochemical analyses on the Lot DUBs and explained how Lot DUBs are different from other OTU-DUBs.
The cytochrome b carboxyl terminal region is necessary for mitochondrial complex III assemblySynthesis/assembly of Cytb, encoded in the mitochondrial DNA, are regulated by the Cytb C-terminus. It modifies the interaction Cytb-Cbp3/Cbp6 to control Cytb and respiratory complex III biogenesis.
New botulinum neurotoxin constructs for treatment of chronic painA new technique to make therapeutic botulinum neurotoxins via isopeptide bonding is described. The novel elongated botulinum neurotoxin can treat nerve injury pain without causing muscle paralysis.
arfA antisense RNA regulates MscL excretory activityBacteria coordinate how they respond to translation and osmotic stress by using antisense RNA crosstalk between the genes involved in the respective stress response pathways.
The role of Limch1 alternative splicing in skeletal muscle functionThis works identifies a novel, alternatively spliced isoform of LIMCH1, that is, developmentally regulated and specific to skeletal muscle. Knocking out the six skeletal muscle–specific exons of LIMCH1 leads to impaired skeletal muscle function in vivo and ex vivo.
Interaction hub critical for telomerase recruitment and primer-template handling for catalysisBy deleting, mutating, and adding back parts of the telomerase enzyme that extends chromosome ends, we uncover features important for its proper functioning in the cell.