Molecular Biology
Biochemical and functional characterization of a meiosis-specific Pch2/ORC AAA+ assemblyThe AAA+ protein Pch2 forms a biochemical complex with Orc1/ORC to suppress DNA break formation in the meiotic G2/prophase.
The RECQL helicase prevents replication fork collapse during replication stressThis work shows that RECQL is an essential player in the protection of stalled replication forks and possibly the restart of broken replication forks, thereby representing a target for cancer therapy.
DDX5 promotes oncogene C3 and FABP1 expressions and drives intestinal inflammation and tumorigenesisIn intestinal epithelial cells (IECs), DDX5 promotes the expression of immune response genes and oncogenes posttranscriptionally and is a novel therapeutic target for treating colitis and intestinal cancers.
Evolutionary divergence reveals the molecular basis of EMRE dependence of the human MCUUsing clues from evolution, chimeric proteins, and biochemical methods, this work identifies a new domain in mitochondrial calcium uniporter that determines its dependence on its binding partner EMRE.
A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cellsThe paralogous COPI coat subunit γ1-COP plays a unique role in promoting neurite outgrowth during the neuronal differentiation of mouse pluripotent cells.
Tradeoff between metabolic i-proteasome addiction and immune evasion in triple-negative breast cancerEngaging the immunoproteasome helps maintain proteostasis during TNBC development, but ultimately leads to increased immune visibility and favorable prognosis. Assessing i-proteasome expression could augment the prognostic and/or predictive value of TILs assessment in diagnostic practice.
The molecular clock protein Bmal1 regulates cell differentiation in mouse embryonic stem cellsAnalysis of the role of the molecular clock protein Bmal1 in pluripotent embryonic stem cells reveals a novel function of circadian proteins in cell differentiation and early embryo development.
The CCR4–NOT complex maintains liver homeostasis through mRNA deadenylationThe CCR4–NOT complex maintains liver homeostasis by fine-tuning levels of mRNAs, including those for transcription factors, cell cycle regulators, DNA damage response proteins, and liver function–related molecules.
A novel class of polymorphic toxins in BacteroidetesBacteria often use toxins to mediate their interaction with the environment. This study reveals a new class of polymorphic toxins, which contain an N-terminal TANFOR domain, in Bacteroidetes.
CSDE1 controls gene expression through the miRNA-mediated decay machineryCSDE1 is a new component of miRNA-induced silencing complex and interacts with DCP1–DCP2 decapping machinery with potential roles in miRNA-mediated target degradation.