Molecular Biology
Evolutionary association of receptor-wide amino acids with G protein–coupling selectivity in aminergic GPCRsEvolutionary analyses of aminergic G protein–coupled receptors reveal receptor-wide potential determinants of G protein–coupling selectivity.
DUSP4 protects BRAF- and NRAS-mutant melanoma from oncogene overdose through modulation of MITFOur study demonstrates that in BRAF- and NRAS-mutant MITF-proficient melanoma, the DUSP4 MAPK phosphatase restricts MAPK activation and prevents oncogenic overdose through modulation of MITF function.
C9ORF72-derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neuronsExtracellularly delivered poly-GAs are internalised into astrocytes via an endocytosis-dependent pathway and then spread to motor neurons, implying non-cell autonomous mechanisms in C9ORF72-ALS/FTD.
Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26This study provides molecular insights into RNA target recognition by the TRIM-NHL protein Mei-P26 that functions in Drosophila ovarian germline stem cell maintenance, oogenesis, and spermatogenesis.
Rab40c regulates focal adhesions and PP6 activity by controlling ANKRD28 ubiquitylationThe role of novel Rab40c/CRL5 ubiquitylation complex in regulating PP6 activity and cell migration.
The miR-26 family regulates early B cell development and transformationWe show that the activity of the miR-26 family determines early B cell behavior: high miR-26 levels promote cell expansion and block the pre-B to immature B cell transition, whereas a miR-26 reduction limits expansion and enhances pre-B cell differentiation.
Phospholipids alter activity and stability of mitochondrial membrane-bound ubiquitin ligase MARCH5This study shows that lipids can act as regulators for the ubiquitination process and can control the stability and activity of a membrane-embedded E3 ubiquitin ligase.
Tricalbin proteins regulate plasma membrane phospholipid homeostasisThe extended synaptotagmin (E-Syt) family are conserved proteins that function at membrane contacts. This study reveals that yeast E-Syt family members regulate phospholipid homeostasis necessary for plasma membrane integrity.
LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reportersDescription of affordable ($40) open-source platform for real-time in-cell monitoring of luciferase activity.
Functional and structural deficiencies of Gemin5 variants associated with neurological disordersGemin5 clinical variants perturbing the dimerization module and the noncanonical RNA-binding site impair oligomerization, RNA–protein interaction networks, ribosome association, and protein stability.