Molecular Biology
Single-cell RNA sequencing of mouse islets exposed to proinflammatory cytokinesSingle-cell RNA-seq was used to show that islet endocrine cells respond to acute cytokine exposure with an increase in the expression of protective genes and the absence of apoptotic gene expression.
Replicated chromatin curtails 53BP1 recruitment in BRCA1-proficient and BRCA1-deficient cellsThis study demonstrates how single cell normalization to genome size provides insight into genome function, here in the context of DNA double-strand break repair by 53BP1 versus BRCA1–BARD1.
The AXL-PYK2-PKCα axis as a nexus of stemness circuits in TNBCA clinically relevant AXL-PYK2-PKCα axis where PYK2 and PKCα act as signaling nodes and functionally cooperate to converge stemness promoting pathways and regulate Oct4 and Nanog pluripotent TFs.
Mapping the micro-proteome of the nuclear lamina and lamina-associated domainsThe nuclear lamina provides structure to the nucleus and serves as an interface between the cytoskeleton and large heterochromatin domains called LADs. This study describes the microproteome of this LAD/lamina interface.
Arterial stiffness and cardiac dysfunction in Hutchinson–Gilford Progeria Syndrome corrected by inhibition of lysyl oxidaseThe findings show that increased lysyl oxidase abundance is causal for the elevated arterial stiffness present in the arteries of Hutchinson–Gilford Progeria Syndrome mice. Pharmacologic inhibition of lysyl oxidase improves cardiac dysfunction and restores arterial compliance.
Concomitant gain and loss of function pathomechanisms in C9ORF72 amyotrophic lateral sclerosisAxonal trafficking deficits and neurodegeneration in C9ORF72 motoneurons are mediated by GOF and LOF mechanisms with RNA foci and DPRs as upstream events, whereas DNA damage appears downstream.
Modulating HSF1 levels impacts expression of the estrogen receptor α and antiestrogen responseThis work shows that activation of the main cellular stress response pathway is responsible for antiestrogen resistance in breast cancer, a process that is reversible.
CDCP1 promotes compensatory renal growth by integrating Src and Met signalingCDCP1 promotes HGF-induced compensatory renal growth by focally and temporally integrating Src and Met-STAT3 signaling in lipid rafts.
Structural comparison of GLUT1 to GLUT3 reveal transport regulation mechanism in sugar porter familyA new structure of GLUT1, compared with GLUT3, can explain substrate affinity differences, and provide a functional rationale for key structural motifs that define the universal Sugar Porter family.
Reduced RNA turnover as a driver of cellular senescenceRNAs originating from transcription upstream and downstream of genes accumulate in the cytoplasm of a subset of senescent cells, suggesting an RNA alternative to cytoplasmic DNA in the triggering of senescence.