Molecular Biology
Characterization of ori and parS-like functions in secondary genome replicons in Deinococcus radioduransAn array of direct repeats upstream to parAB operon helps in secondary genome elements’ maintenance independent of primary chromosome in multipartite genome harboring bacterium Deinococcus radiodurans.
Inhibition of a transcriptional repressor rescues hearing in a splicing factor–deficient mouseThe vital role of the splicing factor SRRM4 in vestibular and inner hair cells of the ear is inactivation of the gene repressor REST; however, in outer hair cells, SRRM4 is dispensable for REST inactivation, which SRRM3 accomplishes independently.
Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeastThe canonical LINC complex is composed of two different transmembrane proteins; this work reveals the heterotrimeric composition of the telomere-associated LINC complex in budding yeast.
Biochemical and functional characterization of a meiosis-specific Pch2/ORC AAA+ assemblyThe AAA+ protein Pch2 forms a biochemical complex with Orc1/ORC to suppress DNA break formation in the meiotic G2/prophase.
The RECQL helicase prevents replication fork collapse during replication stressThis work shows that RECQL is an essential player in the protection of stalled replication forks and possibly the restart of broken replication forks, thereby representing a target for cancer therapy.
DDX5 promotes oncogene C3 and FABP1 expressions and drives intestinal inflammation and tumorigenesisIn intestinal epithelial cells (IECs), DDX5 promotes the expression of immune response genes and oncogenes posttranscriptionally and is a novel therapeutic target for treating colitis and intestinal cancers.
Evolutionary divergence reveals the molecular basis of EMRE dependence of the human MCUUsing clues from evolution, chimeric proteins, and biochemical methods, this work identifies a new domain in mitochondrial calcium uniporter that determines its dependence on its binding partner EMRE.
A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cellsThe paralogous COPI coat subunit γ1-COP plays a unique role in promoting neurite outgrowth during the neuronal differentiation of mouse pluripotent cells.
Tradeoff between metabolic i-proteasome addiction and immune evasion in triple-negative breast cancerEngaging the immunoproteasome helps maintain proteostasis during TNBC development, but ultimately leads to increased immune visibility and favorable prognosis. Assessing i-proteasome expression could augment the prognostic and/or predictive value of TILs assessment in diagnostic practice.
The molecular clock protein Bmal1 regulates cell differentiation in mouse embryonic stem cellsAnalysis of the role of the molecular clock protein Bmal1 in pluripotent embryonic stem cells reveals a novel function of circadian proteins in cell differentiation and early embryo development.