Molecular Biology
Concomitant gain and loss of function pathomechanisms in C9ORF72 amyotrophic lateral sclerosisAxonal trafficking deficits and neurodegeneration in C9ORF72 motoneurons are mediated by GOF and LOF mechanisms with RNA foci and DPRs as upstream events, whereas DNA damage appears downstream.
Modulating HSF1 levels impacts expression of the estrogen receptor α and antiestrogen responseThis work shows that activation of the main cellular stress response pathway is responsible for antiestrogen resistance in breast cancer, a process that is reversible.
CDCP1 promotes compensatory renal growth by integrating Src and Met signalingCDCP1 promotes HGF-induced compensatory renal growth by focally and temporally integrating Src and Met-STAT3 signaling in lipid rafts.
Structural comparison of GLUT1 to GLUT3 reveal transport regulation mechanism in sugar porter familyA new structure of GLUT1, compared with GLUT3, can explain substrate affinity differences, and provide a functional rationale for key structural motifs that define the universal Sugar Porter family.
Reduced RNA turnover as a driver of cellular senescenceRNAs originating from transcription upstream and downstream of genes accumulate in the cytoplasm of a subset of senescent cells, suggesting an RNA alternative to cytoplasmic DNA in the triggering of senescence.
The uncharacterized protein FAM47E interacts with PRMT5 and regulates its functionsThe uncharacterized protein FAM47E interacts with and stabilizes the protein arginine methyltransferase PRMT5, regulating its epigenetic functions thereby modulating target gene expression.
HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tailsCiliary accumulation of G protein–coupled receptors HTR6 and SSTR3 depends on redundant ciliary targeting sequences acting via ciliary trafficking adapters TULP3 and RABL2.
FIREWORKS: a bottom-up approach to integrative coessentiality network analysisA tool to create bias-adjusted coessentiality networks reveals functional relationships between genes, context-specific rewiring of genetic networks, determinants of essentiality, and therapeutic targets for challenging proteins.
ESI mutagenesis: a one-step method for introducing mutations into bacterial artificial chromosomesA simple and efficient recombineering-based method for introducing point mutations into bacterial artificial chromosomes using an artificial intron cassette.
Characterization of ori and parS-like functions in secondary genome replicons in Deinococcus radioduransAn array of direct repeats upstream to parAB operon helps in secondary genome elements’ maintenance independent of primary chromosome in multipartite genome harboring bacterium Deinococcus radiodurans.