Molecular Biology
Structural maintenance of chromosomes (SMC) proteins are required for DNA elimination in ParameciumThis work focuses on two functionally distinct paralogues of SMC4 in Paramecium. SMC4-1 appears to have a classical function as a condensin subunit whereas SMC4-2 is required for developmental DNA elimination process.
Analysis of the indispensable RAD51 cofactor BRCA2 in Naganishia liquefaciens, a Basidiomycota yeastIdentification of the BRCA2 homolog in the Basidiomycota yeast Naganishia liquefaciens reveals the general attributes of fungal BRCA2 and its essential role in Rad51-mediated homologous recombination.
2-Hydroxyglutarate modulates histone methylation at specific loci and alters gene expression via Rph1 inhibitionThis study investigates the genome-wide effects of 2-HG accumulation on chromatin and gene expression and identifies Rph1-dependent and H3K36me3-associated gene repression at silenced loci in yeast.
RNA helicase EIF4A1-mediated translation is essential for the GC responseConditional mouse knock-out models show that EIF4A1, but not its cofactors EIF4B or EIF4H, is essential in B cells for their development, the GC response, and increased translation after activation.
Mitochondrial phosphoproteomes are functionally specialized across tissuesThis study explores tissue-specific mitochondrial proteomes and phosphoproteomes in unprecedented depth, offering valuable insights into mitochondrial organization and specialization.
DOCK1 insufficiency disrupts trophoblast function and pregnancy outcomes via DUSP4-ERK pathwayThis study reveals that decreased DOCK1 in placental villi affects trophoblast function via the DUSP4-ERK pathway, providing insights into adverse pregnancy outcomes.
Cristae dynamics is modulated in bioenergetically compromised mitochondriaLive-cell super-resolution nanoscopy reveals modulation of cristae dynamics in bioenergetically compromised mitochondria demonstrating that cristae morphology and dynamics are intricately connected.
Visualization of conformational transition of GRP94 in solutionSAXS, nsEM, and HDX-MS reveal that one extended and two compact conformations of GRP94 coexist in solution and that the binding and hydrolysis of ATP only drives the transition between the two compact conformations while leaving the extended population unaffected.
DCAF14 regulates CDT2 to promote SET8-dependent replication fork protectionDCAF14 modulates CRL4CDT2-dependent turnover during DNA replication. Loss of DCAF14 causes nascent strand degradation due to excessive proteasomal turnover of SET8 by aberrant CDT2 activity.
Spt5 interacts genetically with Myc and is limiting for brain tumor growth in DrosophilaKnockdown of Spt5 in Drosophila affects various Myc-dependent phenotypes and delays the growth of a Myc-dependent brain tumor, thereby significantly extending the longevity of tumorous animals.