Molecular Biology
Single-domain antibody screening by isPLA-seqThis study describes a high-sensitive, high-throughput single-domain antibody library screening approach, which is applicable for any given interested protein at single-cell resolution by isPLA-seq.
PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islandsPROSER1 promotes the interaction between TET2 and the glycosyltransferase OGT to regulate TET2 O-GlcNAcylation and stability on genomic elements that depend on the activity of the MLL3/4 complexes.
Gut microbiota–derived short-chain fatty acids protect against the progression of endometriosisThis study revealed that gut-derived n-butyrate protects against endometriosis by activating the expression of RAP1GAP GTPase to inhibit the RAP1 oncogenic pathway, indicating n-butyrate is linked with intestinal microbiota and endometriosis disease.
Engineered RNA biosensors enable ultrasensitive SARS-CoV-2 detection in a simple color and luminescence assayThis work reports engineered toehold RNA–based biosensors for COVID-19 diagnostics, with a simple color or luminescence readout that makes it easily deployable in both well-equipped labs as well as low resource settings.
The DREAM complex represses growth in response to DNA damage in ArabidopsisThis work presents composition, associated proteins, and topological aspects of the DREAM complex in plants and links to the DNA damage response by protein interaction data and mutant analysis.
The Th1 cell regulatory circuitry is largely conserved between human and mouseComparison of the binding sites of the Th1 lineage-specifying transcription factor T-bet between human and mouse reveals differences in binding sites but that the most of the target genes are shared.
Quantitative proteomics identifies PTP1B as modulator of B cell antigen receptor signalingThis study analyses the function of the protein tyrosine phosphatase 1B identifying its binding partners and dephosphorylation targets for modulating B cell antigen receptor signaling.
Elevated glucose increases genomic instability by inhibiting nucleotide excision repairExposure to chronic, elevated glucose inhibits nucleotide excision repair, which leads to accumulation of DNA glycation adducts, increased DNA strand breaks, and activation of the DNA damage response.
RNA sequencing reveals niche gene expression effects of beta-hydroxybutyrate in primary myotubesRNA sequencing reveals primary myocyte-specific gene-regulatory niche effects for β-hydroxybutyrate but do not support a general signaling of β-hydroxybutyrate role in other primary cells or during cellular differentiation in vitro.
AMOTL2 mono-ubiquitination by WWP1 promotes contact inhibition by facilitating LATS activationThis work reveals a novel function of WWP1 E3 ligase in the mono-ubiquitination of AMOTL2, which enables the binding and activation of LATS kinases upon contact inhibition.