Molecular Biology
The late-onset Alzheimer’s disease risk factor RHBDF2 is a modifier of microglial TREM2 proteolysisThis study demonstrates that the Alzheimer’s disease risk factor iRhom2/RHBDF2 is a modifier of microglial TREM2 proteolysis and establishes ADAM17 as a physiological TREM2 protease in microglia.
PICH impacts the spindle assembly checkpoint via its DNA translocase and SUMO-interaction activitiesGenome-edited cell lines for PICH depletion or replacement to loss of function mutants revealed an unidentified PICH function impacting SAC activity through modulating Bub1 association at kinetochores.
Unconventional receptor functions and location-biased signaling of the lactate GPCR in the nucleusHCAR1 as GPCR for Lactate is highly expressed in many cancers due to the Warburg effect. We show HCAR1 has a nuclear localization where it promotes cancer malignancy through chromatin regulation, DNA damage repair, translational regulation, and intranuclear signaling.
Assessment of PRMT6-dependent alternative splicing in pluripotent and differentiating NT2/D1 cellsPRMT6, through its methyltransferase activity, contributes to alternative splicing in both pluripotent and neuronally differentiating NT2/D1 cells, affecting primarily cassette exons.
COA5 has an essential role in the early stage of mitochondrial complex IV assemblyThe mitochondrial protein COA5 was previously reported as a complex IV assembly factor. Studying a further case of COA5-related mitochondrial disease, the authors delineate an essential role of this protein in the early stage of complex IV assembly.
lncRNA CARINH regulates expression and function of innate immune transcription factor IRF1 in macrophagesThe antisense lncRNA CARINH acts as an additional regulatory layer of antiviral immunity through coordinated regulation of the transcription factor IRF1 and downstream interferon-stimulated genes.
FrozONE: quick cell nucleus enrichment for comprehensive proteomics analysis of frozen tissuesThe authors establish and benchmark a nuclear proteomics workflow that is faster, simpler, and more scalable than classical protocols, while being as efficient, specific, and reproducible.
4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hostsTo streamline expression construct preparation for screening in diverse expression hosts, we present vectors and protocols using Golden Gate ligation to guide one-step Gibson assembly.
Fatty acid synthase inhibition alleviates lung fibrosis via β-catenin signal in fibroblastsIdiopathic pulmonary fibrosis (IPF) is a fatal disease marked by activated fibroblasts. This study shows fatty acid synthase (FASN) inhibition induces quiescent fibroblasts, reduces β-catenin, and alleviates pulmonary fibrosis, suggesting FASN as a therapeutic target.
