Molecular Biology
- SIRT4 as a novel interactor and candidate suppressor of C-RAF kinase in MAPK signaling
This study proposed a novel extra-mitochondrial role of the tumor suppressor SIRT4 in negatively regulating the MAPK pathway by interacting with C-RAF kinase.
- Correction: The pyruvate dehydrogenase complex regulates mitophagic trafficking and protein phosphorylation
Mutations in the PDC affect the phosphorylation and mitophagic trafficking of matrix proteins, through the novel regulation of associated kinases and a phosphatase. We suggest that this occurs by the direct allosteric regulation of the phosphatase and kinases by the PDC.
- Genetic ablation of Immt induces a lethal disruption of the MICOS complex
A new conditional mouse model deleting Immt/MIC60 reveals the necessity of this protein in vivo and provides an important resource for future research into the mitochondrial MICOS complex.
- Stable structures or PABP1 loading protects cellular and viral RNAs against ISG20-mediated decay
In this work, two novel elements that modulate the susceptibility of target RNAs to degradation by the antiviral RNase ISG20 are described.
- Metabolic and circadian inputs encode anticipatory biogenesis of hepatic fed microRNAs
Unravelling molecular and physiological mechanisms that couple circadian and metabolic cues to regulate anticipatory miR biogenesis during fed-fast-refed cycles in the liver.
- Variety in the USP deubiquitinase catalytic mechanism
Quantitative analysis shows that USP family members have varying catalytic triads. Their third and fourth catalytic residues change in role and importance for nucleophilic attack and catalysis.
- Correction: Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish
The vast majority (99%) of mitochondrial proteins are encoded by the nuclear genome, synthesized in the cytosol and imported into the organelle. Here we study the principles of mitochondrial import in vivo from the RNA perspective using zebrafish.
- Knockout of PA200 improves proteasomal degradation and myelination in a proteotoxic neuropathy
Proteasome Activator 200 is upregulated in a neuropathy model in which protein degradation by the proteasome is impaired. Knocking out PA200 unexpectedly rescued proteostasis and prevented neuropathy.
- Zebrafish tsc1 and cxcl12a increase susceptibility to mycobacterial infection
Knockdown of miR-126 increases susceptibility to mycobacterial infection which can be independently reversed by targeting Tsc1/mTOR or ccr2 implicating macrophage function.
- FERM domain–containing proteins are active components of the cell nucleus
The information we gathered from the literature reveals that FERM domain proteins perform important and diverse functions not only in the cytoplasm, but also in the cell nucleus.