Molecular Biology
C9ORF72-derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neuronsExtracellularly delivered poly-GAs are internalised into astrocytes via an endocytosis-dependent pathway and then spread to motor neurons, implying non-cell autonomous mechanisms in C9ORF72-ALS/FTD.
Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26This study provides molecular insights into RNA target recognition by the TRIM-NHL protein Mei-P26 that functions in Drosophila ovarian germline stem cell maintenance, oogenesis, and spermatogenesis.
Rab40c regulates focal adhesions and PP6 activity by controlling ANKRD28 ubiquitylationThe role of novel Rab40c/CRL5 ubiquitylation complex in regulating PP6 activity and cell migration.
The miR-26 family regulates early B cell development and transformationWe show that the activity of the miR-26 family determines early B cell behavior: high miR-26 levels promote cell expansion and block the pre-B to immature B cell transition, whereas a miR-26 reduction limits expansion and enhances pre-B cell differentiation.
Phospholipids alter activity and stability of mitochondrial membrane-bound ubiquitin ligase MARCH5This study shows that lipids can act as regulators for the ubiquitination process and can control the stability and activity of a membrane-embedded E3 ubiquitin ligase.
LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reportersDescription of affordable ($40) open-source platform for real-time in-cell monitoring of luciferase activity.
Tricalbin proteins regulate plasma membrane phospholipid homeostasisThe extended synaptotagmin (E-Syt) family are conserved proteins that function at membrane contacts. This study reveals that yeast E-Syt family members regulate phospholipid homeostasis necessary for plasma membrane integrity.
Functional and structural deficiencies of Gemin5 variants associated with neurological disordersGemin5 clinical variants perturbing the dimerization module and the noncanonical RNA-binding site impair oligomerization, RNA–protein interaction networks, ribosome association, and protein stability.
All-trans retinoic acid changes muscle fiber type via increasing GADD34 dependent on MAPK signalATRA increases GADD34 expression by decreasing the expression of Six1, which down-regulates the transcriptional activity with TLE3 and increasing mRNA stability through blocking the interaction between TTP and ARE on GADD34 mRNA, resulting in muscle fiber type change.
Mre11 exonuclease activity promotes irreversible mitotic progression under replication stressMre11 exonuclease facilitates the processing of stalled replication forks upon mitotic entry, leading to irreversible mitotic progression and mitotic replisome disassembly.