Molecular Biology
Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic KrasG12D in pancreatic cancerThis work identifies the use of exosomes to specifically deliver CRISPR/Cas9 to target oncogenic KrasG12D mutation in pancreatic cancer as a nonviral therapeutic strategy.
Heterogeneous nuclear ribonucleoprotein E1 binds polycytosine DNA and monitors genome integrityhnRNP E1 binds polycytosine tracts of DNA and monitors genome integrity.
Long noncoding RNA HOTAIR interacts with Y-Box Protein-1 (YBX1) to regulate cell proliferationThe authors determined that HOTAIR specifically bind to YBX1 and promote its nuclear translocation, and then regulating cell proliferation by stimulating the PI3K/Akt and ERK/RSK signaling pathways.
Microglia show differential transcriptomic response to Aβ peptide aggregates ex vivo and in vivoExogenous amyloid beta peptides cause distinct transcriptomic responses in microglia cultures that are not fully replicated in an amyloid mouse model or identified microglial subpopulations in vivo.
A role for condensin in mediating transcriptional adaptation to environmental stimuliFast conditional depletion of the chromosomal condensin complex from budding yeast cells reveals roles in transcriptional regulation, especially during the establishment of new gene expression patterns.
Single-cell RNA sequencing of mouse islets exposed to proinflammatory cytokinesSingle-cell RNA-seq was used to show that islet endocrine cells respond to acute cytokine exposure with an increase in the expression of protective genes and the absence of apoptotic gene expression.
Replicated chromatin curtails 53BP1 recruitment in BRCA1-proficient and BRCA1-deficient cellsThis study demonstrates how single cell normalization to genome size provides insight into genome function, here in the context of DNA double-strand break repair by 53BP1 versus BRCA1–BARD1.
The AXL-PYK2-PKCα axis as a nexus of stemness circuits in TNBCA clinically relevant AXL-PYK2-PKCα axis where PYK2 and PKCα act as signaling nodes and functionally cooperate to converge stemness promoting pathways and regulate Oct4 and Nanog pluripotent TFs.
Mapping the micro-proteome of the nuclear lamina and lamina-associated domainsThe nuclear lamina provides structure to the nucleus and serves as an interface between the cytoskeleton and large heterochromatin domains called LADs. This study describes the microproteome of this LAD/lamina interface.
Arterial stiffness and cardiac dysfunction in Hutchinson–Gilford Progeria Syndrome corrected by inhibition of lysyl oxidaseThe findings show that increased lysyl oxidase abundance is causal for the elevated arterial stiffness present in the arteries of Hutchinson–Gilford Progeria Syndrome mice. Pharmacologic inhibition of lysyl oxidase improves cardiac dysfunction and restores arterial compliance.