Microbiology, Virology & Host Pathogen Interaction
Prognostic accuracy of MALDI-TOF mass spectrometric analysis of plasma in COVID-19High mortality rate in SARS-CoV-2–infected individuals requires accurate markers for predicting COVID-19 severity. MALDI-TOF analysis revealed differential plasma levels of SAA1 and SAA2 associated with higher risk of hospitalization and can be used to improve COVID-19 monitoring and therapy.
Vaccinia virus hijacks ESCRT-mediated multivesicular body formation for virus egressPoxvirus extracellular virions are critical for virus virulence. This study shows that multivesicular bodies serve as a major cellular source of membrane for their formation and spread.
Profiling chromatin accessibility responses in human neutrophils with sensitive pathogen detectionATAC-seq reveals unique neutrophil chromatin architecture changes in response to different stimuli before transcriptional activation, possibly regulating downstream gene expression.
Viperin interacts with PEX19 to mediate peroxisomal augmentation of the innate antiviral responseThis work highlights the multifunctional role of the ISG viperin and its interaction with the peroxisomal protein Pex19 to modulate peroxisomal-dependent innate signaling that ultimately restricts viral infection.
Human IFITM3 restricts chikungunya virus and Mayaro virus infection and is susceptible to virus-mediated counteractionEndogenous and heterologpusly expressed human IFITM3 restricts early and late replication steps of chikungunya and Mayaro virus infection and is downregulated at the protein level in infected cells.
Cell-autonomous Toxoplasma killing program requires Irgm2 but not its microbe vacuolar localizationIrgm2 controls Toxoplasma killing via selective recruitment of Gbp1 and Irgb6 to Toxoplasma parasitophorous vacuoles, whereas its vacuolar localization is dispensable for parasite killing.
Cell-free expression of the outer membrane protein OprF of Pseudomonas aeruginosa for vaccine purposesProduction of recombinant proteoliposomes containing OprF from P. aeruginosa promotes the active open conformation of the porin exposing native epitopes. These OprF proteoliposomes were used as vaccines to protect mice against a P. aeruginosa acute pulmonary infection model.
Crystal structure of inhibitor-bound human MSPL that can activate high pathogenic avian influenzaThe structure of extracellular domain of MSPL and inhibitor complex helps to understand the TTSP functions, including TMPRSS2, and provides the insights of the infection of influenza and SARS-CoV.
DGCR8 deficiency impairs macrophage growth and unleashes the interferon response to mycobacteriaLack of miRNA biosynthesis in DGCR8-deficient macrophages did not block macrophage differentiation but caused overshooting type I IFN responses to mycobacterial glycolipids.
Phylo-geo-network and haplogroup analysis of 611 novel coronavirus (SARS-CoV-2) genomes from IndiaEvolution of SARS-CoV-2 in India across 51 haplogroups based on 152 parsimony informative sites revealed B6 and B1 (Pangolin) and A2a (Covidex) as the most prevalent lineage and clade, respectively.