Microbiology, Virology & Host Pathogen Interaction
Tyrosine phosphorylation controlled poly(A) polymerase I activity regulates general stress response in bacteriaWe show a tyrosine phosphorylation–controlled poly(A) polymerase I polyadenylation activity regulated stress response mechanism in bacteria that is distinct and operates downstream of the primary stress regulator RpoS.
Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identityLeptospira strains show very large antigenic variation (serovars). Here, the rfb gene cluster is shown to be transferred horizontally within/among Leptospira species, discriminating serovar identity.
Pandemic Vibrio cholerae acquired competitive traits from an environmental Vibrio speciesPandemic-associated Vibrio cholerae interbacterial defense genes were likely acquired through competition with and subsequent gene transfer from the fish pathogen Vibrio anguillarum.
Discovery of RUF6 ncRNA–interacting proteins involved in P. falciparum immune evasionAn RNA-directed proteomic discovery (ChIRP-MS) protocol is used to identify in vivo RUF6 ncRNA–protein interactions that sustain virulence genes in the human malaria parasite, Plasmodium falciparum.
Genome-wide profiling of histone modifications in Plasmodium falciparum using CUT&RUNWe present a detailed step-wise protocol for reliable genome-wide profiling of histone modifications using CUT&RUN that requires only low numbers of Plasmodium falciparum blood stages.
The potential of a universal influenza virus-like particle vaccine expressing a chimeric cytokineHerein, a virus-like particle vaccine with chimeric cytokine shows the protective efficacy against different strains of influenza virus, laying the foundation for designing a universal influenza vaccine.
Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replicationGenetic evidence and in vitro experiments with live virus suggest EXOSC2 depletion can reduce SARS-CoV-2 replication without significant toxicity.
Contribution of the Golgi apparatus in morphogenesis of a virus-induced cytopathic vacuolar systemElectron tomography reveals four classes of cytopathic vesicles-II (CPV-II) stemming from the host Golgi apparatus after Venezuelan equine encephalitis virus infection.
Binding stoichiometry and structural model of the HIV-1 Rev/importin β complexBiochemical, biophysical, and docking studies provide insights into how the HIV-1 regulatory protein Rev is recognized by the nuclear import factor importin beta.
Efficacy of SARS-CoV-2 vaccination in patients with monoclonal gammopathies: A cross sectional studyUninfected multiple myeloma patients do not develop adequate immunity after SARS-COV-2 vaccination and will clearly benefit from tailored monitoring of immune responses after booster vaccine doses.