Microbiology, Virology & Host Pathogen Interaction
Caspase-4 dimerisation and D289 auto-processing elicit an interleukin-1β-converting enzymeThe noncanonical inflammasome generates p32/p9 and p34/p9 active caspase-4 species via dimerisation and autocleavage; both species cleave GSDMD to induce pyroptosis, whereas p34/p9 matures IL-1β.
High-resolution kinetic characterization of the RIG-I-signaling pathway and the antiviral responseThis study takes a comprehensive approach to understand the dynamics of the cell-intrinsic response towards virus infection and how viruses actively modulate this.
Disulfide stabilization reveals conserved dynamic features between SARS-CoV-1 and SARS-CoV-2 spikesSARS-CoV-1 spike protein is stabilized with engineered disulfide bonds, and cryo-EM imaging of these stabilized S-trimers reveals rare spike conformations.
SLIT2/ROBO1 signaling suppresses mTORC1 for organelle control and bacterial killingSLIT2, a canonical neurorepellent, promotes lysosome biogenesis and augments autophagic flux to modulate immune responses in macrophages. In cancer cells, SLIT2 negatively regulates mTORC1 signaling to control cell growth.
STING is redundant for host defense and pathology of COVID-19-like disease in miceSTING-deficient mice infected with SARS-CoV-2 do not show altered disease development or viral load, nor any major differences in inflammatory responses.
Amphid sensory neurons of Caenorhabditis elegans orchestrate its survival from infection with broad classes of pathogensThis study shows that the nervous system of the bacterivorous nematode Caenorhabditis elegans coordinates pathogen-specific and broad immune responses during infection.
Expression of TMPRSS2 is up-regulated by bacterial flagellin, LPS, and Pam3Cys in human airway cellsThe expression of the virus-activating protease TMPRSS2 in airway cells is enhanced by bacterial components. This may contribute to virus activation and replication in co-infections of IAV and bacteria.
Spatial metabolomics for symbiotic marine invertebratesMass spectrometry imaging revealed in situ spatial metabolite distribution patterns at 50 μm resolution for sea anemones and scleractinian corals and their microalgal endosymbionts.
Structural insights into ubiquitin chain cleavage by Legionella ovarian tumor deubiquitinasesLegionella possesses OTU deubiquitinases (Lot DUBs), which hinder the host ubiquitin system. We performed structural and biochemical analyses on the Lot DUBs and explained how Lot DUBs are different from other OTU-DUBs.
The cytochrome b carboxyl terminal region is necessary for mitochondrial complex III assemblySynthesis/assembly of Cytb, encoded in the mitochondrial DNA, are regulated by the Cytb C-terminus. It modifies the interaction Cytb-Cbp3/Cbp6 to control Cytb and respiratory complex III biogenesis.