Microbiology, Virology & Host Pathogen Interaction
Elevation in sphingolipid upon SARS-CoV-2 infection: possible implications for COVID-19 pathologySARS-CoV-2 infection alters the levels of sphingolipids early post infection. This phenomenon is reflected by increased levels of sphingolipids, including gangliosides, in infected cells, as well as in serum in a SARS-CoV-2 murine model.
Structural basis of membrane recognition of Toxoplasma gondii vacuole by Irgb6Upon Toxoplasma gondii infection, Irgb6 is recruited to the parasitophorous vacuole membrane (PVM) where Irgb6 disrupts it. We solved the atomic structures of Irgb6 in two distinct nucleotide states, revealing a unique PVM-binding interface sensitive to GTPase cycling.
Structure of Aedes aegypti procarboxypeptidase B1 and its binding with Dengue virus for controlling infectionUsing high-resolution structure, we have characterized the substrate specificity of Aedes aegypti procarboxypeptidase B1 and provided mechanistic insights into the binding and inhibition of DENV
Gut microbiota–derived short-chain fatty acids protect against the progression of endometriosisThis study revealed that gut-derived n-butyrate protects against endometriosis by activating the expression of RAP1GAP GTPase to inhibit the RAP1 oncogenic pathway, indicating n-butyrate is linked with intestinal microbiota and endometriosis disease.
An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasitesMalaria arises during the proliferation of Plasmodium spp. in human blood, whereas the underlying atypical cell division mechanisms remain poorly understood. This study uses advanced imaging to dissect dynamics and organization of the centrosome, a key division regulator.
Autoimmune anti-DNA and anti-phosphatidylserine antibodies predict development of severe COVID-19COVID-19 induces high levels of autoimmune anti-DNA and anti-phosphatidylserine antibodies that are detected in some patients upon hospital admission and predict later development of severe disease.
Hepatitis B virus compartmentalization and single-cell differentiation in hepatocellular carcinomaSingle-cell RNA-Seq unravels heterogeneity and compartmentalization of both hepatitis B virus and cancer identifying new candidate pathways for viral hepatocarcinogenesis.
A serum proteome signature to predict mortality in severe COVID-19 patientsA serum proteome panel, which includes several type-3 cystatins and inter-α-trypsin inhibitors, predicts mortality in severe COVID-19 patients very well, in three independent cohorts.
Prognostic accuracy of MALDI-TOF mass spectrometric analysis of plasma in COVID-19High mortality rate in SARS-CoV-2–infected individuals requires accurate markers for predicting COVID-19 severity. MALDI-TOF analysis revealed differential plasma levels of SAA1 and SAA2 associated with higher risk of hospitalization and can be used to improve COVID-19 monitoring and therapy.
Vaccinia virus hijacks ESCRT-mediated multivesicular body formation for virus egressPoxvirus extracellular virions are critical for virus virulence. This study shows that multivesicular bodies serve as a major cellular source of membrane for their formation and spread.