Microbiology, Virology & Host Pathogen Interaction
Cell-free expression of the outer membrane protein OprF of Pseudomonas aeruginosa for vaccine purposesProduction of recombinant proteoliposomes containing OprF from P. aeruginosa promotes the active open conformation of the porin exposing native epitopes. These OprF proteoliposomes were used as vaccines to protect mice against a P. aeruginosa acute pulmonary infection model.
Crystal structure of inhibitor-bound human MSPL that can activate high pathogenic avian influenzaThe structure of extracellular domain of MSPL and inhibitor complex helps to understand the TTSP functions, including TMPRSS2, and provides the insights of the infection of influenza and SARS-CoV.
DGCR8 deficiency impairs macrophage growth and unleashes the interferon response to mycobacteriaLack of miRNA biosynthesis in DGCR8-deficient macrophages did not block macrophage differentiation but caused overshooting type I IFN responses to mycobacterial glycolipids.
Phylo-geo-network and haplogroup analysis of 611 novel coronavirus (SARS-CoV-2) genomes from IndiaEvolution of SARS-CoV-2 in India across 51 haplogroups based on 152 parsimony informative sites revealed B6 and B1 (Pangolin) and A2a (Covidex) as the most prevalent lineage and clade, respectively.
SARS-CoV-2 infection induces protective immunity and limits transmission in Syrian hamstersUsing an aged Syrian hamster model, which develops severe morbidity upon SARS-CoV-2 infection including death, we demonstrated that the immunity induced by a natural infection not only protects hamsters from reinfection but also significantly limits transmission via close contact.
Profiling of immune dysfunction in COVID-19 patients allows early prediction of disease progressionDeep immune profiling of COVID-19 patients with different disease severity reveals populations associated with severe disease and can be used to predict disease severity early on.
Sustained Helicobacter pylori infection accelerates gastric dysplasia in a mouse modelHp infection was found to alter metaplasia and accelerate dysplasia in mice. Thus, Hp can promote gastric preneoplastic progression, in addition to its known role in initiating chronic inflammation.
Inhibiting the reproduction of SARS-CoV-2 through perturbations in human lung cell metabolic networkUsing genomic and structural information from SARS-CoV-2, we created a biomass function capturing its amino and nucleic acid requirements and incorporated this into a metabolic model of the human lung cell to predict metabolic perturbations that inhibit virus reproduction.
G-CSF shifts erythropoiesis from bone marrow into spleen in the setting of systemic inflammationGranulocyte colony-stimulating factor suppresses erythropoiesis of bone marrow and promotes splenic erythropoiesis during lipopolysaccharide-induced systemic inflammation in mice.
Characterization of ori and parS-like functions in secondary genome replicons in Deinococcus radioduransAn array of direct repeats upstream to parAB operon helps in secondary genome elements’ maintenance independent of primary chromosome in multipartite genome harboring bacterium Deinococcus radiodurans.