Microbiology, Virology & Host Pathogen Interaction
An evolutionary approach to systematic discovery of novel deubiquitinases, applied to LegionellaThe analysis of the relationships between different deubiquitinase classes leads to the definition of an aromatic “gatekeeper” motif that distinguishes DUBs from other cysteine proteases and helps to predict new bacterial DUBs.
TMPRSS2 and furin are both essential for proteolytic activation of SARS-CoV-2 in human airway cellsThe spike protein S of SARS-CoV-2 is activated by TMPRSS2 and furin. Inhibition of either one of these proteases can render the S protein unable to mediate virus entry and, therefore, provides a promising approach for COVID-19 treatment.
Structural basis of HapEP88L-linked antifungal triazole resistance in Aspergillus fumigatusThe mutation P88L in subunit HapE of the CCAAT transcription factor causes resistance of Aspergillus fumigatus to azole drugs due to altered binding, bending, and transcription of target promoters.
Paneth cell α-defensin misfolding correlates with dysbiosis and ileitis in Crohn’s disease model miceThis study provides novel insight into Crohn’s disease where α-defensin misfolding resulting from excessive ER stress in Paneth cells induces dysbiosis and disease progression.
Simultaneous multiple allelic replacement in the malaria parasite enables dissection of PKG functionThis work combines the use of an inducible Cre recombinase and different lox sites to simultaneously create multiple allelic replacements. We show that an essential malarial cGMP-dependent protein kinase functions exclusively during the release of the parasite from its host cell.
A novel class of polymorphic toxins in BacteroidetesBacteria often use toxins to mediate their interaction with the environment. This study reveals a new class of polymorphic toxins, which contain an N-terminal TANFOR domain, in Bacteroidetes.
The health status alters the pituitary function and reproduction of mice in a Cxcr2-dependent mannerThis study explores the effects of microbiota on reproductive function of Cxcr2 knockout animals. Cxcr2 is involved in the control of pituitary action and the subsequent development of mammary gland, uterus and ovary.
Gammaherpesvirus-infected germinal center cells express a distinct immunoglobulin repertoireGerminal center B cells infected with gammaherpesvirus display altered repertoire with biased usage of lambda light chain and skewed utilization of IGHV genes.
Virus-induced transposable element expression up-regulation in human and mouse host cellsGenome-wide transposon expression up-regulation in host cells regardless of virus, species, and host cell tissue types occurs early during viral infection and likely contributes to promoting the host innate immune response.
A key region of molecular specificity orchestrates unique ephrin-B1 utilization by Cedar virusAn expanded hydrophobic cavity within the structurally constrained receptor-binding site of the Cedar virus attachment glycoprotein facilitates idiosyncratic utilization of ephrin-B1.