Methods & Resources
Exploratory meta-analysis of hypoxic transcriptomes using a precise transcript reference sequence setExploratory meta-analysis of hypoxic RNA-seq data using FANTOM-CAT as a reference transcriptome facilitates the evaluation of relationship between sense–antisense transcripts from the same locus.
Cdh5-lineage–independent origin of dermal lymphatics shown by temporally restricted lineage tracingTemporally restricted lineage tracing reveals a non-venous source of dermal lymphatic vessels and highlights Cre induction strategy as a critical parameter for stage-specific cell labeling.
Improving the computation efficiency of polygenic risk score modeling: faster in JuliaTo enable computationally efficient polygenic risk score (PRS) calculations, PRS.jl translates a field standard PRS construction method, PRS-CS, to the Julia programming language.
Multi-omics profiling identifies a deregulated FUS-MAP1B axis in ALS/FTD–associated UBQLN2 mutantsAnalysis of ALS patient-derived and engineered cells revealed that mutant UBQLN2 increases mRNA and protein of MAP1B which is mediated by dephosphorylation of FUS within its RNA-binding domain.
Subgenomic RNA profiling suggests novel mechanism in coronavirus gene regulation and host adaptionCORONATATOR, developed to analyze the subgenomic RNA of coronavirus, was applied on large number of sequencing data sets from coronaviruses, and shed light on viral gene regulation.
LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reportersDescription of affordable ($40) open-source platform for real-time in-cell monitoring of luciferase activity.
Evaluation of the correctable decoding sequencing as a new powerful strategy for DNA sequencingThis article proposed the correctable decoding sequencing technology with conservative theoretical error rate of 0.0009%, and evaluated its robustness by simulation. This technology can provide a powerful new protocol for NGS platforms, enabling accurate identification of rare mutations in medicine.
Tracking connectivity maps in human stem cell–derived neuronal networks by holographic optogeneticsHolographic optogenetic stimulation of human iPSC–derived neuronal networks was exploited to map precise functional connectivity motifs and their long-term dynamics during network development.
Sensitive visualization of SARS-CoV-2 RNA with CoronaFISHWe design an RNA-FISH method to target the plus and minus strands of SARS-CoV-2 RNA, and apply it to image the virus in cell lines, lung tissue, and nasal swabs, by fluorescence and electron microscopy.
SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heartIn a unique model of fluorescent based mapping of EV recipient cells, RBC-EVs were found to signal to cardiac cells and regulate gene expression in a model of ischemic heart failure.