Methods & Resources
A protocol for single nucleus RNA-seq from frozen skeletal muscleThe authors detail a method to isolate nuclei for single nucleus RNA sequencing from frozen, biobanked human skeletal muscle samples.
Efficient knock-in method enabling lineage tracing in zebrafishThis study introduces a knock-in method in zebrafish that is cloning-free, preserves the endogenous gene, achieves high germline transmission, and useful for both cell labelling and lineage tracing.
New algorithms for accurate and efficient de novo genome assembly from long DNA sequencing readsInnovative algorithmic approaches were used to perform assembly of complex genomes across the tree of life, from long DNA sequencing data.
Activation and inhibition of the C-terminal kinase domain of p90 ribosomal S6 kinasesThe design of a constitutively active form of the C-terminal kinase domain of the p90 ribosomal S6 kinase 2 (RSK2) is described, and this is based on a phosphomimetic mutation and a C-terminal truncation, yielding a high kinase activity.
A genome-wide CRISPR functional survey of the human phagocytosis molecular machineryThe validation and interpretation of a genome-wide CRISRPko FACS screen via protein–protein interaction data yield a broad view of the molecular network-regulating phagocytosis in human cells.
Loss of surface transport is a main cellular pathomechanism of CRB2 variants causing podocytopathiesCRB2 is an essential protein of the renal filtration barrier. This study reports the development of an in vitro assay, suitable to investigate the pathogenic potential of known and novel, so far uncharacterized CRB2 variants.
Drosophila pVALIUM10 TRiP RNAi lines cause undesired silencing of Gateway-based transgenesDrosophila pVALIUM10 TRiP RNAi lines cause undesirable knockdown of transgenic reporters and overexpression lines generated with the help of the Gateway cloning technology by targeting attB sites.
Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents usedMono(ADP-ribosyl)ation is an emerging modification, for which recently detection reagents were generated. We cross-validated all reagents and observe remarkable differences in efficiency, depending on the substrate investigated and on the methods that are used.
In vivo probing of SECIS-dependent selenocysteine translation in ArchaeaBy turning a bacterial reporter into an archaeal selenoprotein, in vivo probing of structure–function relations during UGA recoding for selenoprotein synthesis in Archaea is greatly facilitated.
A high-content endogenous GLUT4 trafficking assay reveals new aspects of adipocyte biologyThe authors describe a high-throughput method for measuring endogenous GLUT4 and transferrin receptor translocation in adipocytes. They reveal that this method has advantages over studying GLUT4 trafficking using overexpressed GLUT4 reporters, which are commonly used in the field.