Methods & Resources
High-quality single-cell transcriptomics from ovarian histological sections during folliculogenesisQuantitative RNA-sequencing method for single cells isolated from alcohol- and formalin-fixed tissue sections discovers new relationships between histology and transcriptome during folliculogenesis.
A targeted sequencing extension for transcript genotyping in single-cell transcriptomicsWe describe the targeted sequencing extension and provide two proof-of-concept examples of how it can assist with understanding the impact of genetic variants.
Refining the genetic risk of breast cancer with rare haplotypes and pattern miningA closed-pattern mining algorithm helps identify rare, noncontiguous haplotypes underlying breast cancer risk in British women in the UK Biobank.
Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopeA cohort of low-abundance transmembrane proteins concentrated at the nuclear envelope has been identified, predicting diverse new functions for this membrane system.
Time-resolved proteomic analyses of senescence highlight metabolic rewiring of mitochondriaTime-resolved proteomic analyses of senescent fibroblasts reveal that nearly 40% of the analyzed mitochondrial proteome have changed upon induction of senescence. Our study highlights the rewiring of mitochondrial metabolism as a central feature among all changes in mitochondrial pathways of senescent cells.
Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generationThis article describes a new method to evaluate the actomyosin activity in living cardiomyocytes during beating based on nonlinear scattering microscopy, which will be useful for elucidating cardiomyopathy mechanisms, efficacy of an intervention, and others.
BiFCo: visualizing cohesin assembly/disassembly cycle in living cellsA novel strategy developed in fission yeast to visualise only-assembled cohesin complex loading/unloading cycles in eukaryotic chromosomes in living cells.
Gapless provides combined scaffolding, gap filling, and assembly correction with long readsgapless is a new tool for combined execution of correction, scaffolding and gap filling using PacBio or Oxford Nanopore reads.
Rapid and precise genotyping of transgene zygosity in mice using an allele-specific methodA novel, precise, rapid, and generalizable method to determine transgene zygosity in mice by allele-specific restriction enzyme digestion of PCR products from transgenes or homologous endogenous genes.
A novel approach to measure complex V ATP hydrolysis in frozen cell lysates and tissue homogenatesThis novel method can be used in clinical samples to determine CV function in diseases, with the added benefit of being able to use frozen samples in a high-throughput manner and to explore ATP hydrolysis as a drug target for disease treatment.