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Methods & Resources

  • Ribosomal stalling landscapes revealed by high-throughput inverse toeprinting of mRNA libraries
    Open Access
    Ribosomal stalling landscapes revealed by high-throughput inverse toeprinting of mRNA libraries

    Britta Seip, Guénaël Sacheau, Denis Dupuy, C Axel Innis

    Britta Seip ... C Axel Innis

    Published 9 October 2018

    High-throughput inverse toeprinting identifies peptide-encoding transcripts that induce ribosome stalling and allows the systematic analysis of sequence-dependent translational events.

  • Distinctive features of lincRNA gene expression suggest widespread RNA-independent functions
    Open Access
    Distinctive features of lincRNA gene expression suggest widespread RNA-independent functions

    Alex C Tuck, Kedar Nath Natarajan, Greggory M Rice, Jason Borawski, Fabio Mohn, Aneliya Rankova, Matyas Flemr, Alice Wenger, Razvan Nutiu, Sarah Teichmann, Marc Bühler

    Alex C Tuck ... Marc Bühler

    Published 31 July 2018

    Combining single-cell RNA-seq of mouse ESCs and NPCs, lincRNA gene deletions, conditional RNA depletion, and nuclear exosome profiling distinguishes RNA-dependent and RNA-independent lincRNA gene activities.

  • Silica-based solid-phase extraction of cross-linked nucleic acid–bound proteins
    Open Access
    Silica-based solid-phase extraction of cross-linked nucleic acid–bound proteins

    Claudio Asencio, Aindrila Chatterjee, Matthias W Hentze

    Claudio Asencio ... Matthias W Hentze

    Published 18 June 2018

    The 2C method allows the rapid and straightforward isolation of nucleic acid–protein complexes, greatly simplifying downstream applications for the study of DNA– and RNA–protein interactions.

  • Multiple membrane extrusion sites drive megakaryocyte migration into bone marrow blood vessels
    Open Access
    Multiple membrane extrusion sites drive megakaryocyte migration into bone marrow blood vessels

    Edward Brown, Leo M Carlin, Claus Nerlov, Cristina Lo Celso, Alastair W Poole

    Edward Brown ... Alastair W Poole

    Published 21 May 2018

    Megakaryocytes enter bone marrow sinusoids to generate platelets by extrusion of internal membrane into the plasma membrane at fusion sites rather than microtubule-driven proplatelet extension.

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