Methods & Resources
Genetic ablation of Immt induces a lethal disruption of the MICOS complexA new conditional mouse model deleting Immt/MIC60 reveals the necessity of this protein in vivo and provides an important resource for future research into the mitochondrial MICOS complex.
Ageing-dependent thiol oxidation reveals early oxidation of proteins with core proteostasis functionsProteome-wide analysis of thiol oxidation during chronological ageing of yeast reveals evolutionarily conserved proteins as early-oxidation targets with a potential impact on proteostasis regulation.
Extreme trait GWAS (Et-GWAS): Unraveling rare variants in the 3,000 rice genomeUsing extreme bulk pooling and allele frequency assessment, the graphical user interface (GUI)-enabled association technique successfully revealed relationships between genetic variations and grain yield under drought stress condition.
RedRibbon: A new rank–rank hypergeometric overlap for gene and transcript expression signaturesRedRibbon is a comparative analysis tool of differential omics analyses to reveal overlapping features between two differential studies, with high performance, accuracy, and simplicity in use.
Mitochondrial phosphoproteomes are functionally specialized across tissuesThis study explores tissue-specific mitochondrial proteomes and phosphoproteomes in unprecedented depth, offering valuable insights into mitochondrial organization and specialization.
CRISPR/Cas9 bioluminescence-based assay for monitoring CFTR trafficking to the plasma membraneA novel CRISPR/Cas9-based bioluminescence system to assess total and live-cell plasma membrane WT-CFTR levels offers a versatile platform for new screening systems in CFTR modulator discovery.
Automated staging of zebrafish embryos with deep learningIntroducing KimmelNet: A deep learning model that accurately stages populations of zebrafish embryos from 2D brightfield images, offering a rapid and objective alternative to manual observations.
MICA: a multi-omics method to predict gene regulatory networks in early human embryosOur comparative analysis of gene regulatory network predictions defines a pipeline to infer transcription factor–target gene regulatory interactions in early human embryos. Mutual information refined by chromatin accessibility allowed us to construct the first network during early human development.
Assessing MAPPs assay as a tool to predict the immunogenicity potential of protein therapeuticsThis work highlights the use of streptavidin magnetic beads as a valuable approach for the immunoaffinity purification of mass spectrometry MHCII-eluted ligands (MAPPs) and their practical application in assessing the drug-induced immunogenicity risk of novel biotherapeutics.
Cost-effective DNA methylation profiling by FML-seqA low-cost, high-throughput method for genome-wide DNA methylation profiling based on methylation-specific restriction digestion and sequencing.