Methods & Resources
Novel imaging tools to study mitochondrial morphology in Caenorhabditis elegansThis study presents novel genetic tools for live imaging of mitochondrial morphology in the nematode Caenorhabditis elegans, offering insights into mitochondrial dynamics and providing a thorough comparison with existing methods.
A scalable approach to topic modelling in single-cell data by approximate pseudobulk projectionRandom projection of pseudobulk gene expression data improves the scalability and accuracy of single-cell topic modelling.
pyRBDome: a comprehensive computational platform for enhancing RNA-binding proteome dataThis study explores enhancing protein–RNA interaction prediction using machine learning.
Nanobodies as novel tools to monitor the mitochondrial fission factor Drp1This study reports the first nanobodies against the mitochondrial fission protein Drp1 for application in proteomics, high-resolution microscopy and live cell imaging.
REMEMProt: a resource of membrane-enriched proteome profiles, their disease associations, and biomarker statusA resource of experimental specific membrane-enriched mass spectrometry–derived proteome.
iMPAQT reveals that adequate mitohormesis from TFAM overexpression leads to life extension in miceOverexpressing TFAM leads to smaller, leaner mice with extended lifespans. The iMPAQT analysis revealed changes in mitochondrial protein expression and characteristic metabolic shifts indicative of mitohormesis as an adaptive strategy in skeletal muscle.
Combining sophisticated fast FLIM, confocal microscopy, and STED nanoscopy for live-cell imaging of tunneling nanotubesHere, we proposed an innovative advanced light imaging to reveal the dynamics of tunneling nanotubes (TNTs) that are now considered as a long- and short-distance route for cell-to-cell communication.
Reverse-engineering the anti-MUC1 antibody 139H2 by mass spectrometry–based de novo sequencingA widely used anti-MUC1 antibody (139H2) is sequenced by mass spectrometry, revealing the molecular basis of its glycosylation-independent binding to this tumor-associated antigen.
Genetic ablation of Immt induces a lethal disruption of the MICOS complexA new conditional mouse model deleting Immt/MIC60 reveals the necessity of this protein in vivo and provides an important resource for future research into the mitochondrial MICOS complex.
Ageing-dependent thiol oxidation reveals early oxidation of proteins with core proteostasis functionsProteome-wide analysis of thiol oxidation during chronological ageing of yeast reveals evolutionarily conserved proteins as early-oxidation targets with a potential impact on proteostasis regulation.