Methods & Resources
Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generationThis article describes a new method to evaluate the actomyosin activity in living cardiomyocytes during beating based on nonlinear scattering microscopy, which will be useful for elucidating cardiomyopathy mechanisms, efficacy of an intervention, and others.
BiFCo: visualizing cohesin assembly/disassembly cycle in living cellsA novel strategy developed in fission yeast to visualise only-assembled cohesin complex loading/unloading cycles in eukaryotic chromosomes in living cells.
Gapless provides combined scaffolding, gap filling, and assembly correction with long readsgapless is a new tool for combined execution of correction, scaffolding and gap filling using PacBio or Oxford Nanopore reads.
Rapid and precise genotyping of transgene zygosity in mice using an allele-specific methodA novel, precise, rapid, and generalizable method to determine transgene zygosity in mice by allele-specific restriction enzyme digestion of PCR products from transgenes or homologous endogenous genes.
A novel approach to measure complex V ATP hydrolysis in frozen cell lysates and tissue homogenatesThis novel method can be used in clinical samples to determine CV function in diseases, with the added benefit of being able to use frozen samples in a high-throughput manner and to explore ATP hydrolysis as a drug target for disease treatment.
A protocol for single nucleus RNA-seq from frozen skeletal muscleThe authors detail a method to isolate nuclei for single nucleus RNA sequencing from frozen, biobanked human skeletal muscle samples.
Efficient knock-in method enabling lineage tracing in zebrafishThis study introduces a knock-in method in zebrafish that is cloning-free, preserves the endogenous gene, achieves high germline transmission, and useful for both cell labelling and lineage tracing.
New algorithms for accurate and efficient de novo genome assembly from long DNA sequencing readsInnovative algorithmic approaches were used to perform assembly of complex genomes across the tree of life, from long DNA sequencing data.
Activation and inhibition of the C-terminal kinase domain of p90 ribosomal S6 kinasesThe design of a constitutively active form of the C-terminal kinase domain of the p90 ribosomal S6 kinase 2 (RSK2) is described, and this is based on a phosphomimetic mutation and a C-terminal truncation, yielding a high kinase activity.
A genome-wide CRISPR functional survey of the human phagocytosis molecular machineryThe validation and interpretation of a genome-wide CRISRPko FACS screen via protein–protein interaction data yield a broad view of the molecular network-regulating phagocytosis in human cells.