Metabolism
- Hepatic DKK1-driven steatosis is CD36 dependent
Increased DKK1 in hepatic steatosis contributes CD36-mediated fatty acid uptake and insulin resistance.
- Palmitate impairs circadian transcriptomics in muscle cells through histone modification of enhancers
The disruption of circadian rhythms because of lipid overload may lead to epigenomic changes that influence metabolism. Thus, a dietary or therapeutic modulation of lipid levels, a cornerstone in the treatment of metabolic disorders, may prevent circadian misalignment in peripheral tissues.
- A high-content endogenous GLUT4 trafficking assay reveals new aspects of adipocyte biology
The authors describe a high-throughput method for measuring endogenous GLUT4 and transferrin receptor translocation in adipocytes. They reveal that this method has advantages over studying GLUT4 trafficking using overexpressed GLUT4 reporters, which are commonly used in the field.
- An inducible amphipathic α-helix mediates subcellular targeting and membrane binding of RPE65
Amino acid residues 107–125 of RPE65, in concert with a palmitoyl group at C112, form a membrane-sensing amphipathic helix that targets RPE65 to smooth endoplasmic reticulum to acquire its substrate.
- Gain-of-function genetic screens in human cells identify SLC transporters overcoming environmental nutrient restrictions
The authors present a CRISPRa-based gain-of-function approach to systematically uncover solute carriers sustaining cellular fitness upon limiting amino acid concentrations.
- Mitochondrial impairment and intracellular reactive oxygen species alter primary cilia morphology
This work shows how altering energetic status and promoting intracellular/mitochondrial ROS induces cell-dependent ciliary impairments, which is relevant in diseases characterized by these features.
- Methionine uptake via the SLC43A2 transporter is essential for regulatory T-cell survival
Regulatory T cells survive after IL-2 withdrawal by taking up and using methionine through the SLC43A2 transporter in a Notch1-dependent manner.
- Cross talk between glucose metabolism and immunosuppression in IFN-γ–primed mesenchymal stem cells
This study reveals a novel relationship between mesenchymal stem cell immunomodulation and metabolism and provides a new strategy to improve their therapeutic efficacy in inflammatory diseases.
- Mitochondrial stress-induced GFRAL signaling controls diurnal food intake and anxiety-like behavior
Muscle mitochondrial stress signals to the brain via hindbrain-specific GFRAL receptor signaling to promote hypothalamic CRH induction, which associates with GFRAL-dependent modulation of systemic energy metabolism, diurnal food intake, and anxiety-like behavior.
- Islet Gene View—a tool to facilitate islet research
Islet Gene View (IGW) is a web resource that makes information on gene expression in human islets from donors easily accessible to the scientific community. The relationship of global RNA expression from 188 donor-islets with islet phenotypes is explored.