Medical Research
A protocol for single nucleus RNA-seq from frozen skeletal muscleThe authors detail a method to isolate nuclei for single nucleus RNA sequencing from frozen, biobanked human skeletal muscle samples.
Plasma proteome dynamics of COVID-19 severity learnt by a graph convolutional network of multi-scale topologyWe used a graph convolutional network to model the plasma proteome of 384 COVID-19 patients. The model incorporates clinical data and is able to predict severity. We identified the proteins that shifted most in expression depending on severity.
Lung macrophages utilize unique cathepsin K–dependent phagosomal machinery to degrade intracellular collagenThis study provides a comprehensive map of how peritoneal, lung, and brain tissue environment shapes phagosomal composition, revealing cathepsin K as the main peptidase in the intracellular destruction of collagen, which is mainly active in lung macrophages.
Disease- and sex-specific differences in patients with heart valve disease: a proteome studyProteomics provide novel insights into molecular mechanisms and cardiac remodeling in heart failure because of pressure overload in aortic valve stenosis and volume overload in mitral valve regurgitation.
Mutation-agnostic RNA interference with engineered replacement rescues Tmc1-related hearing lossMutation-agnostic RNA interference with concomitant, knockdown-resistant gene replacement, results in robust auditory brainstem response and cochlear hair cell preservation in the murine model of TMC1-related autosomal dominant hearing loss.
Kindlin2 enables EphB/ephrinB bi-directional signaling to support vascular developmentKindlin2 supports EphB4/ephrinB2 bi-directional signaling functions by associating with a highly conserved NIYY motif in the ephrinB2 cytoplasmic tail to promote ephrinB2 clustering and EphB4 activation, thereby enabling arteriovenous segregation during embryonic development.
Transcriptome analyses in infertile men reveal germ cell–specific expression and splicing patternsWe uncover extensive germ cell type–dependent transcriptome changes in human spermatogenesis and demonstrate the potential of alternative splicing in the regulation of spermatogenesis.
Tanshinone functions as a coenzyme that confers gain of function of NQO1 to suppress ferroptosisNQO1 catalyzes the reduction of tanshinones to detoxify lipid peroxyl radicals and inhibit ferroptosis both in vitro and in vivo, providing a molecular mechanism for the therapeutic effects of tanshinones, such as cardiac protection.
A prediction model for COVID-19 liver dysfunction in patients with normal hepatic biochemical parametersThe composite model of sex and the novel protein marker ANGL3 exhibits great potential in predicting the risk of developing LD in COVID-19 patients with normal hepatic biochemical parameters on admission.
Mitochondrial stress-induced GFRAL signaling controls diurnal food intake and anxiety-like behaviorMuscle mitochondrial stress signals to the brain via hindbrain-specific GFRAL receptor signaling to promote hypothalamic CRH induction, which associates with GFRAL-dependent modulation of systemic energy metabolism, diurnal food intake, and anxiety-like behavior.