Medical Research
Inhibition of microRNA-33b in humanized mice ameliorates nonalcoholic steatohepatitisExperiments using humanized mice created by knocking in microRNA (miR)-33b into an intron of Srebf1 show that suppression of miR-33b by nucleic acid drugs is useful for the treatment of NASH.
Intranasal soluble ACE2 improves survival and prevents brain SARS-CoV-2 infectionA soluble ACE2 protein bioengineered to have extended duration of action and enhanced affinity for SARS-CoV-2 provides markedly improved survival and organ protection when administered intranasally. Reducing brain SARS-CoV-2 titers is an important determinant of therapeutic efficacy.
Cell death and barrier disruption by clinically used iodine concentrationsClinically used concentrations of povidone-iodine (PVP-I) are toxic to eukaryotic cells by attacking the lipid bilayer and tight junctions with fast kinetics and urge the reappraisal of PVP-I use.
A protocol for single nucleus RNA-seq from frozen skeletal muscleThe authors detail a method to isolate nuclei for single nucleus RNA sequencing from frozen, biobanked human skeletal muscle samples.
Plasma proteome dynamics of COVID-19 severity learnt by a graph convolutional network of multi-scale topologyWe used a graph convolutional network to model the plasma proteome of 384 COVID-19 patients. The model incorporates clinical data and is able to predict severity. We identified the proteins that shifted most in expression depending on severity.
Lung macrophages utilize unique cathepsin K–dependent phagosomal machinery to degrade intracellular collagenThis study provides a comprehensive map of how peritoneal, lung, and brain tissue environment shapes phagosomal composition, revealing cathepsin K as the main peptidase in the intracellular destruction of collagen, which is mainly active in lung macrophages.
Disease- and sex-specific differences in patients with heart valve disease: a proteome studyProteomics provide novel insights into molecular mechanisms and cardiac remodeling in heart failure because of pressure overload in aortic valve stenosis and volume overload in mitral valve regurgitation.
Mutation-agnostic RNA interference with engineered replacement rescues Tmc1-related hearing lossMutation-agnostic RNA interference with concomitant, knockdown-resistant gene replacement, results in robust auditory brainstem response and cochlear hair cell preservation in the murine model of TMC1-related autosomal dominant hearing loss.
Kindlin2 enables EphB/ephrinB bi-directional signaling to support vascular developmentKindlin2 supports EphB4/ephrinB2 bi-directional signaling functions by associating with a highly conserved NIYY motif in the ephrinB2 cytoplasmic tail to promote ephrinB2 clustering and EphB4 activation, thereby enabling arteriovenous segregation during embryonic development.
Transcriptome analyses in infertile men reveal germ cell–specific expression and splicing patternsWe uncover extensive germ cell type–dependent transcriptome changes in human spermatogenesis and demonstrate the potential of alternative splicing in the regulation of spermatogenesis.