Growth and the Environment of Schizosaccharomyces pombe
- 1Flinders University, Flinders Centre for Innovation in Cancer, School of Medicine, FMST, Bedford Park, Adelaide, South Australia 5042, Australia;
- 2Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037
Abstract
Here, we summarize the composition and uses of Schizosaccharomyces pombe media and discuss key issues for consideration in the generation of S. pombe cultures. We discuss the concept of “culture memory,” in which the growth state and stress experienced by a strain during storage, propagation, and starter culture preparation can alter experimental outcomes at later stages. We also describe the triggers that are widely used to manipulate signaling through the environment sensing pathways.
Footnotes
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↵3 Correspondence: janni.petersen{at}flinders.edu.au; prussell{at}scripps.edu
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From the Fission Yeast collection, edited by Iain M. Hagan, Antony M. Carr, Agnes Grallert, and Paul Nurse.
- © 2016 Cold Spring Harbor Laboratory Press