A TIN2 dyskeratosis congenita mutation causes telomerase-independent telomere shortening in mice

Abstract

The progressive bone marrow failure syndrome dyskeratosis congenita (DC) is often caused by mutations in telomerase or the factors involved in telomerase biogenesis and trafficking. However, a subset of DC patients is heterozygous for mutations in the shelterin component TIN2. To determine how the TIN2-DC mutations affect telomere function, we generated mice with the equivalent of the TIN2 K280E DC allele (TIN2^DC) by gene targeting. Whereas homozygous TIN2^DC/DC mice were not viable, first-generation TIN2^+/DC mice were healthy and fertile. In the second and third generations, the TIN2^+/DC mice developed mild pancytopenia, consistent with hematopoietic dysfunction in DC, as well as diminished fecundity. Bone marrow telomeres of TIN2^+/DC mice shortened over the generations, and immortalized TIN2^+/DC mouse embryonic fibroblasts (MEFs) showed telomere shortening with proliferation. Unexpectedly, telomere shortening was accelerated in TIN2^+/DC mTR^−/− mice and MEFs compared with TIN2^+/+ mTR^−/− controls, establishing that the TIN2^DC telomere maintenance defect was not solely due to diminished telomerase action. The TIN2^DC allele induced mild ATR kinase signaling at telomeres and a fragile telomere phenotype, suggestive of telomere replication problems. These data suggest that this TIN2-DC mutation could induce telomeric dysfunction phenotypes in telomerase-negative somatic cells and tissues that further exacerbate the telomere maintenance problems in telomerase-positive stem cell compartments.