A novel, noncanonical mechanism of cytoplasmic polyadenylation operates in Drosophila embryogenesis
- 1Gene Regulation Programme, Centre de Regulació Genòmica (CRG-UPF), 08003 Barcelona, Spain;
- 2Bioinformatics Programme, Centre de Regulació Genòmica (CRG-UPF), 08003 Barcelona, Spain
Abstract
Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation that requires two sequences in the 3′ untranslated region (UTR) of vertebrate substrates: the polyadenylation hexanucleotide, and the cytoplasmic polyadenylation element (CPE). Using a cell-free Drosophila system, we show that these signals are not relevant for Toll polyadenylation but, instead, a “polyadenylation region” (PR) is necessary. Competition experiments indicate that PR-mediated polyadenylation is required for viability and is mechanistically distinct from the CPE/hexanucleotide-mediated process. These data indicate that Toll mRNA is polyadenylated by a noncanonical mechanism, and suggest that a novel machinery functions for cytoplasmic polyadenylation during Drosophila embryogenesis.
Keywords
Footnotes
-
↵3 Corresponding author.
E-MAIL fatima.gebauer{at}crg.es; FAX 34-93-3969983.
-
Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.568610.
-
Supplemental material is available at http://www.genesdev.org.
-
- Received July 20, 2009.
- Accepted November 23, 2009.
- Copyright © 2010 by Cold Spring Harbor Laboratory Press
