We have developed an inducible system to visualize gene expression at the levels of DNA,
RNA and protein in living cells. The system is composed of a 200 copy transgene array …

Proteins are often referred to in accordance with the activity with which they were first
associated or the organelle in which they were initially identified. However, a variety of nuclear …

RNA processing events that take place on the transcribed pre-mRNA include capping,
splicing, editing, 3′ processing, and polyadenylation. Most of these processes occur co-…

Understanding gene expression requires the ability to follow the fate of individual molecules.
Here we use a cellular system for monitoring messenger RNA (mRNA)expression to …

We imaged transcription in living cells using a locus-specific reporter system, which allowed
precise, single-cell kinetic measurements of promoter binding, initiation and elongation. …

Live-cell single mRNA imaging is a powerful tool but has been restricted in higher eukaryotes
to artificial cell lines and reporter genes. We describe an approach that enables live-cell …

The four-dimensional nucleome (4DN) consortium studies the architecture of the genome
and the nucleus in space and time. We summarize progress by the consortium and highlight …

Activin A, a cytokine member of the transforming growth factor-β superfamily, is expressed
locally by the mesenchymal component of the hemopoietic microenvironment. Its expression …

The flow of genetic information in eukaryotic cells occurs through the nucleocytoplasmic
translocation of mRNAs. Knowledge of in vivo messenger RNA export kinetics remains poor in …

4078 localized to the leading edge of the cells (Lawrence and Singer, 1986). This correlates
with the elevated levels of ßactin protein required in lamellipodia, which depend on the …