Despite key roles in sister chromatid cohesion and chromosome organization, the
mechanism by which cohesin rings are loaded onto DNA is still unknown. Here we combine …

The cohesin complex topologically encircles DNA to promote sister chromatid cohesion.
Alternatively, cohesin extrudes DNA loops, thought to reflect chromatin domain formation. Here, …

Loop extrusion has emerged as a prominent hypothesis for how SMC complexes shape
chromosomes – single molecule in vitro observations have yielded fascinating images of this …

Cohesin forms a proteinaceous ring that is thought to link sister chromatids by entrapping
DNA and counteracting the forces generated by the mitotic spindle. Whether individual …

Background Sister chromatids are held together by the ring-shaped cohesin complex, which
is loaded onto chromosomes before DNA replication. Cohesion between sister …

Post-replicative correction of replication errors by the mismatch repair (MMR) system is critical
for suppression of mutations. Although the MMR system may need to handle nucleosomes …

The ring-shaped chromosomal cohesin complex holds sister chromatids together by
topological embrace, a prerequisite for accurate chromosome segregation. Cohesin plays …

The loading of cohesin onto chromatin requires the heterodimeric complex sister chromatid
cohesion (Scc)2 and Scc4 (Scc2/4), which is highly conserved in all species. Here, we …

… Torahiko Higashi as first author – report a potential mechanism for how cohesin forms DNA
loops in vitro based on cryo-electron microscopy and biochemical observations (Higashi et al.…

… Immortal hepatic HEp-G2 Lucia AhR cells (InvivoGen, San Diego, CA, USA) with or without
CtL2 infection were cultured in the presence or absence of L-kynurenine (LK) with or without …