Positioned nucleosomes limit the access of proteins to DNA and implement regulatory
features encoded in eukaryotic genomes. Here we have generated the first genome-wide …

Topoisomerases are involved in many aspects of DNA metabolism such as replication and
transcription reactions. Camptothecins, which stabilize the covalent intermediate of …

Cell fate specification relies on the action of critical transcription factors that become
available at distinct stages of embryonic development. One such factor is NeuroD1, which is …

The genomes of higher eukaryotes are carefully balanced systems of gene expression that
compensate for the different numbers of sex chromosomes in the two sexes by adjusting …

T-cell–recruiting bispecific molecule therapy has yielded promising results in patients with
hematologic malignancies; however, resistance and subsequent relapse remains a major …

Histone variants are emerging as key regulatory molecules in cancer. We report a unique
role for the H2A.Z isoform H2A.Z.2 as a driver of malignant melanoma. H2A.Z.2 is highly …

Dosage compensation in male Drosophila relies on the X chromosome–specific recruitment
of a chromatin-modifying machinery, the dosage compensation complex (DCC). The …

Unique functions of mammalian DNA-topoisomerases IIα and -β are suggested by their distinct
cellular distribution and chromatin binding at mitosis. Here, we studied H69-VP cells that, …

Chromatin immunoprecipitation (ChIP) is widely used to identify chromosomal binding sites.
Chromatin proteins are cross-linked to their target sequences in living cells. The purified …

For the compact Drosophila genome, several factors mediating insulator function, such as su(Hw)
and dCTCF, have been identified. Recent analyses showed that both these insulator‐…