Structure of the FoxM1 DNA-recognition domain bound to a promoter sequence

…, A Stein, RG Hibbert, T Heidebrecht… - Nucleic acids …, 2010 - academic.oup.com
FoxM1 is a member of the Forkhead family of transcription factors and is implicated in
inducing cell proliferation and some forms of tumorigenesis. It binds promoter regions with a …

[HTML][HTML] Glucosylated hydroxymethyluracil, DNA base J, prevents transcriptional readthrough in Leishmania

…, A Haydock, G Ramasamy, S Vainio, T Heidebrecht… - Cell, 2012 - cell.com
… , indicates that J is synthesized in two steps: in the first step, T residues at specific positions
in DNA are hydroxylated to form hydroxymethyluracil (HOMeU); in the second step, a glucose …

Posttranslational modification of microtubules by the MATCAP detyrosinase

…, OB Bleijerveld, J Nieuwenhuis, T Heidebrecht… - Science, 2022 - science.org
The detyrosination-tyrosination cycle involves the removal and religation of the C-terminal
tyrosine of α-tubulin and is implicated in cognitive, cardiac, and mitotic defects. The vasohibin–…

[HTML][HTML] Steroid binding to Autotaxin links bile salts and lysophosphatidic acid signalling

…, R Bolier, D Tolenaars, A Kremer, T Heidebrecht… - Nature …, 2016 - nature.com
… where F t is the observed fluorescence signal relative to the choline product at each time
point (t), F 0 is the background fluorescence signal at the start of each measurement, v max and …

Target highlights in CASP14: Analysis of models by structure providers

…, CS Hayes, T Heidebrecht… - Proteins: Structure …, 2021 - Wiley Online Library
The biological and functional significance of selected Critical Assessment of Techniques for
Protein Structure Prediction 14 (CASP14) targets are described by the authors of the …

Rational design of autotaxin inhibitors by structural evolution of endogenous modulators

WJ Keune, F Potjewyd, T Heidebrecht… - Journal of medicinal …, 2017 - ACS Publications
Autotaxin produces the bioactive lipid lysophosphatidic acid (LPA) and is a drug target of
considerable interest for numerous pathologies. We report the expedient, structure-guided …

[HTML][HTML] Lysophosphatidic acid produced by autotaxin acts as an allosteric modulator of its catalytic efficiency

…, A Fish, MT Matsoukas, T Heidebrecht… - Journal of Biological …, 2018 - ASBMB
Autotaxin (ATX) is a secreted glycoprotein and the only member of the ectonucleotide
pyrophosphatase/phosphodiesterase family that converts lysophosphatidylcholine (LPC) into …

Crystal structure of the tubulin tyrosine carboxypeptidase complex VASH1–SVBP

A Adamopoulos, L Landskron, T Heidebrecht… - Nature Structural & …, 2019 - nature.com
The cyclic enzymatic removal and ligation of the C-terminal tyrosine of α-tubulin generates
heterogeneous microtubules and affects their functions. Here we describe the crystal and …

Expression of protein complexes using multiple Escherichia coli protein co-expression systems: a benchmarking study

D Busso, Y Peleg, T Heidebrecht, C Romier… - Journal of Structural …, 2011 - Elsevier
Escherichia coli (E. coli) remains the most commonly used host for recombinant protein
expression. It is well known that a variety of experimental factors influence the protein production …

The structural basis for recognition of base J containing DNA by a novel DNA binding domain in JBP1

T Heidebrecht, E Christodoulou… - Nucleic acids …, 2011 - academic.oup.com
T-containing equivalent GGCAGC T GCAACAA. A common complementary oligonucleotide
was synthesized labeled in the 5′ with tetramethylrhodamine. Both complementary pairs of …