The silent mating loci and chromosomal regions adjacent to telomeres of S. cerevisiae have
features similar to heterochromatin of more complex eukaryotes. Transcriptional repression …

The factors that sequester transcriptionally repressed heterochromatin at the nuclear
periphery are currently unknown. In a genome-wide RNAi screen, we found that depletion of S-…

We have developed a novel technique for combined immunofluorescence/in situ hybridization
on fixed budding yeast cells that maintains the three-dimensional structure of the nucleus …

Cells have evolved sophisticated multi-protein complexes that can regulate gene activity at
various steps of the transcription process. Recent advances highlight the role of nuclear …

Heterochromatin is characterized by dimethylated or trimethylated histone H3 Lys9 (H3K9me2
or H3K9me3, respectively) and is found at transposable elements, satellite repeats and …

The budding yeast INO80 complex is a conserved ATP-dependent nucleosome remodeler
containing actin-related proteins Arp5 and Arp8. Strains lacking INO80, ARP5, or ARP8 have …

Chromatin structure has a crucial role in processes of metabolism, including transcription,
DNA replication and DNA damage repair. An evolutionarily conserved variant of histone H2A, …

We present a new, robust, computational procedure for tracking fluorescent markers in time-lapse
microscopy. The algorithm is optimized for finding the time-trajectory of single particles …

Telomeric TG-rich repeats and their associated proteins protect the termini of eukaryotic
chromosomes from end-to-end fusions. Associated with the cap structure at yeast telomeres is a …

Little is known about the dynamics of chromosomes in interphase nuclei. By tagging four
chromosomal regions with a green fluorescent protein fusion to lac repressor, we monitored the …