Naïve and primed pluripotent states retain distinct molecular properties, yet limited knowledge
exists on how their state transitions are regulated. Here, we identify Mettl3, an N 6 -…

The flow of genetic information in eukaryotic cells occurs through the nucleocytoplasmic
translocation of mRNAs. Knowledge of in vivo messenger RNA export kinetics remains poor in …

We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional
kinetics of single alleles in real time, using single-gene integrations. We obtained high-…

10.7554/eLife.16748.001 Signal propagation from the cell membrane to a promoter can
induce gene expression. To examine signal transmission through sub-cellular compartments …

Advanced prostate cancer remains incurable and is the second leading cause of mortality in
men. Immunotherapy based on the adoptive transfer of tumor-infiltrating lymphocytes (TIL) …

Adoptive cell therapy (ACT) of autologous tumor infiltrating lymphocytes (TIL) is an effective
immunotherapy for patients with solid tumors, yielding objective response rates of around 40…

Transcription kinetics of actively transcribing genes in vivo have generally been measured
using tandem gene arrays. However, tandem arrays do not reflect the endogenous state of …

Background Targeting epitopes derived from neo-antigens (or “neo-epitopes”) represents a
promising immunotherapy approach with limited off-target effects. However, most peptides …

Imaging of transcription by quantitative fluorescence-based techniques allows the examination
of gene expression kinetics in single cells. Using a cell system for the in vivo visualization …

The stochastic process of gene expression is commonly controlled at the level of RNA
transcription. The synthesis of messenger RNA (mRNA) is a multistep process, performed by RNA …