… In the course of his investigation into the cause of this disaster, Burnet isolated S. aureus …
Many S. aureus isolates produce only small amounts of alpha-toxin, and its total absence in S. …

… presence of proteins or cholesterol in the membranes (S. Bhakdi et al., unpublished results).
… fatty acids; however, it is possible that the fatty acids stabilize the membrane interaction (S. …

and Conclusions Depending on the size of the pores one wishes to produce in plasma
membranes, the choice will probably fall on one of the three toxins discussed above. S. aureus α-…

… Study day 0 was defined as the calendar day during which the patient’s temperature fell
and stayed below 37.8C. The study protocol was approved by the Ministry of Public Health (…

Streptolysin-O (SLO) is a thiol-activated, membrane-damaging protein toxin of Mr 69,000 that
is produced by most strains of beta-hemolytic group A streptococci. Native, primarily water-…

… Cells were then scraped off in the presence of lysis buffer and sonicated five times for 5 s
on ice. Thereafter, the lysates (1 mg/ml) were incubated with 1 μg/ml CS546 and 20 μM UDP[ …

… The protein sedimented in a symmetrical peak of ∼5,5 S, and no material was detected in …
HDL was subject to the same enzyme treatment (Bhakdi S, unpublished data, 1998). Binding …

… Subsequently, the newly synthesized first-strand cDNA was subjected to 20 rounds of PCR
amplification of 95C for 40 s, 62C for 1 min, and 72C for 3 min. Reaction mixtures contained …

Agents that deplete cells of K+ without grossly disrupting the plasma membrane were found
to stimulate the cleavage of pro‐interleukin (IL)‐1 beta to mature IL‐1 beta. Agents …

… LTAs from S. aureus and S. pneumoniae failed to induce monokine production when
applied in the concentration range of 0.05 to 5.0 micrograms/ml. However, LTAs from several …