User profiles for R. Nutiu
Razvan NutiuNovartis Institutes for BioMedical Research Verified email at novartis.com Cited by 4153 |
Aptamers with fluorescence-signaling properties
Aptamers are single-stranded DNA or RNA molecules with ligand-binding capabilities.
Signaling aptamers refer to aptamers or modified aptamers with recordable signal generation …
Signaling aptamers refer to aptamers or modified aptamers with recordable signal generation …
Structure-switching signaling aptamers
Aptamers are single-stranded nucleic acids with defined tertiary structures for selective binding
to target molecules. Aptamers are also able to bind a complementary DNA sequence to …
to target molecules. Aptamers are also able to bind a complementary DNA sequence to …
[PDF][PDF] In vitro selection of structure‐switching signaling aptamers
… The reverse primer contained a ribonucleotide (R) at its 3’-end to create a chimeric
antisense strand prone to NaOH-mediated cleavage, which permitted the isolation of the sense …
antisense strand prone to NaOH-mediated cleavage, which permitted the isolation of the sense …
Structure‐switching signaling aptamers: transducing molecular recognition into fluorescence signaling
The development of aptamer technology considerably broadens the utility of nucleic acids
as molecular recognition elements, because it allows the creation of DNA or RNA molecules …
as molecular recognition elements, because it allows the creation of DNA or RNA molecules …
Direct measurement of DNA affinity landscapes on a high-throughput sequencing instrument
R Nutiu, RC Friedman, S Luo, I Khrebtukova… - Nature …, 2011 - nature.com
… The average sequencing intensity correlated better with Gcn4p fluorescent intensity for
motif-containing clusters than did the intensity of a labeled primer (R 2 = 0.48 versus 0.30), …
motif-containing clusters than did the intensity of a labeled primer (R 2 = 0.48 versus 0.30), …
[HTML][HTML] Pervasive regulatory functions of mRNA structure revealed by high-resolution SHAPE probing
mRNAs can fold into complex structures that regulate gene expression. Resolving such
structures de novo has remained challenging and has limited our understanding of the …
structures de novo has remained challenging and has limited our understanding of the …
Affinity analysis of a protein− aptamer complex using nonequilibrium capillary electrophoresis of equilibrium mixtures
… Using these expressions, we can find the ratio, R, of the two equilibrium fractions: … Wheeler,
Kathryn R. Riley. Silver nanoparticle interactions with glycated and non-glycated human …
Kathryn R. Riley. Silver nanoparticle interactions with glycated and non-glycated human …
Splice site strength–dependent activity and genetic buffering by poly-G runs
Pre-mRNA splicing is regulated through the combinatorial activity of RNA motifs, including
splice sites and splicing regulatory elements. Here we show that the activity of the G-run (…
splice sites and splicing regulatory elements. Here we show that the activity of the G-run (…
Adsorption and covalent coupling of ATP-binding DNA aptamers onto cellulose
… The activity was measured using an anti-ATP structure-switching signaling aptamer
developed by Nutiu and Li. In this approach, illustrated in Figure 1, a duplex is made from an …
developed by Nutiu and Li. In this approach, illustrated in Figure 1, a duplex is made from an …
Basestock oils for lubricants from mixtures of corn oil and synthetic diesters
Environmentally friendly vegetable oils and their derivatives represent alternatives to
mineral-based lubricants. Vegetable oils have high biodegradability and low production costs. …
mineral-based lubricants. Vegetable oils have high biodegradability and low production costs. …