Constitutive proteasomes and immunoproteasomes shape the peptide repertoire presented
by major histocompatibility complex class I (MHC-I) molecules by harboring different sets of …

The core particle (CP) of the yeast proteasome is composed of four heptameric rings of subunits
arranged in a hollow, barrel-like structure. We report that the CP is autoinhibited by the N…

The crystal structure of the 20S proteasome from the yeast Saccharomyces cerevisiae shows
that its 28 protein subunits are arranged as an (α1...α7, β1...β7) 2 complex in four stacked …

▪ Abstract Proteasomes are large multisubunit proteases that are found in the cytosol, both
free and attached to the endoplasmic reticulum, and in the nucleus of eukaryotic cells. Their …

The dipeptide boronic acid bortezomib, also termed VELCADE ® , is a proteasome inhibitor
now in use for the treatment of multiple myeloma, and its use for the treatment of other …

Principles of intracellular protein synthesis and protein degradation remain to be among the
most challenging questions of modern cell biology. Proteolysis plays an important role in …

The 436-amino acid protein enolase 1 from yeast was degraded in vitro by purified wild-type
and mutant yeast 20S proteasome particles. Analysis of the cleavage products at different …

Targeted protein degradation is largely performed by the ubiquitin–proteasome pathway, in
which substrate proteins are marked by covalently attached ubiquitin chains that mediate …

In recent years, proteasome inhibitor development has received considerable interest given
the critical role of the proteasome in intracellular processes such as cell cycle progression, …

We present a biochemical and crystallographic characterization of active site mutants of the
yeast 20S proteasome with the aim to characterize substrate cleavage specificity, subunit …