User profiles for Masahito Ikawa

Masahito Ikawa

Professor, Research Institute for Microbial Diseases, Osaka University
Verified email at biken.osaka-u.ac.jp
Cited by 32973

Engineered CRISPR-Cas9 nuclease with expanded targeting space

…, M Seki, H Hirano, H Aburatani, R Ishitani, M Ikawa… - Science, 2018 - science.org
The RNA-guided endonuclease Cas9 cleaves its target DNA and is a powerful genome-editing
tool. However, the widely used Streptococcus pyogenes Cas9 enzyme (SpCas9) …

[HTML][HTML] Green mice'as a source of ubiquitous green cells

M Okabe, M Ikawa, K Kominami, T Nakanishi… - FEBS letters, 1997 - Elsevier
The green fluorescent protein (GFP) is responsible for the green bioluminescence of the
jellyfish Aequorea victoria. Many classes of GFP mutants exist that display modified …

The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs

N Inoue, M Ikawa, A Isotani, M Okabe - Nature, 2005 - nature.com
Representing the 60 trillion cells that build a human body, a sperm and an egg meet,
recognize each other, and fuse to form a new generation of life. The factors involved in this …

A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA

G Tiscornia, O Singer, M Ikawa… - Proceedings of the …, 2003 - National Acad Sciences
We describe the use of lentiviral vectors expressing small interfering RNAs (siRNAs) to
knock down the expression of specific genes in vitro and in vivo. A lentiviral vector capable of …

DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes

…, K Gotoh, Y Totoki, A Toyoda, M Ikawa… - Genes & …, 2008 - genesdev.cshlp.org
Silencing of transposable elements occurs during fetal gametogenesis in males via de novo
DNA methylation of their regulatory regions. The loss of MILI (miwi-like) and MIWI2 (mouse …

Mili, a mammalian member of piwi family gene, is essential for spermatogenesis

…, T Kimura, TW Ijiri, T Isobe, N Asada, Y Fujita, M Ikawa… - 2004 - journals.biologists.com
The piwi family genes, which are defined by conserved PAZ and Piwi domains, play important
roles in stem cell self-renewal, RNA silencing, and translational regulation in various …

Innate versus learned odour processing in the mouse olfactory bulb

…, R Kobayakawa, H Matsumoto, Y Oka, T Imai, M Ikawa… - Nature, 2007 - nature.com
The mammalian olfactory system mediates various responses, including aversive behaviours
to spoiled foods and fear responses to predator odours. In the olfactory bulb, each …

Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos

A Pfeifer, M Ikawa, Y Dayn… - Proceedings of the …, 2002 - National Acad Sciences
The introduction of foreign genes into early mouse embryos and embryonic stem (ES) cells
is invaluable for the analysis of gene function and regulation in the living animal. The use of …

PGC7/Stella protects against DNA demethylation in early embryogenesis

…, T Kimura, H Taniguchi, T Sekimoto, M Ikawa… - Nature cell …, 2007 - nature.com
DNA methylation is an important means of epigenetic gene regulation 1 , 2 and must be
carefully controlled as a prerequisite for normal early embryogenesis. Although global …

[HTML][HTML] Generating green fluorescent mice by germline transmission of green fluorescent ES cells

AK Hadjantonakis, M Gertsenstein, M Ikawa… - Mechanisms of …, 1998 - Elsevier
Green fluorescent protein (GFP) and its variants currently represent the only non-invasive
markers available for labeling mammalian cells in culture or in a multicellular organism …