Next-generation sequencing technology is a powerful tool for transcriptome analysis.
However, under certain conditions, only a small amount of material is available, which requires …

RNA interference (RNAi) is a mechanism by which double-stranded RNAs (dsRNAs) suppress
specific transcripts in a sequence-dependent manner. dsRNAs are processed by Dicer to …

Germ cell fate in mice is induced in pluripotent epiblast cells in response to signals from
extraembryonic tissues. The specification of approximately 40 founder primordial germ cells and …

Genome-wide epigenetic reprogramming in mammalian germ cells, zygote and early embryos,
plays a crucial role in regulating genome functions at critical stages of development. We …

Polycomb-group (Pc-G) genes are required for the stable repression of the homeotic selector
genes and other developmentally regulated genes, presumably through the modulation of …

Germ cell fate in mice is induced in proximal epiblast cells by the extra-embryonic ectoderm,
and is not acquired through the inheritance of any preformed germ plasm. To determine …

Mouse primordial germ cells (PGCs) undergo sequential epigenetic changes and genome-wide
DNA demethylation to reset the epigenome for totipotency. Here, we demonstrate that …

Specification of primordial germ cells (PGCs) marks the beginning of the totipotent state.
However, without a tractable experimental model, the mechanism of human PGC (hPGC) …

Genetic and epigenetic mechanisms regulate the transition from the totipotent zygote to
pluripotent primitive ectoderm cells in the inner cell mass of mouse blastocysts. These …

A unique feature of the germ cell lineage is the generation of totipotency. A critical event in
this context is DNA demethylation and the erasure of parental imprints in mouse primordial …