Biochemical basis of DNA replication fidelity
MF Goodman, S Creighton, LB Bloom… - Critical reviews in …, 1993 - Taylor & Francis
DNA polymerase is the critical enzyme maintaining genetic integrity during DNA replication.
Individual steps in the replication process that contribute to DNA synthesis fidelity include …
Individual steps in the replication process that contribute to DNA synthesis fidelity include …
[19] Gel fidelity assay measuring nucleotide misinsertion, exonucleolytic proofreading, and lesion bypass efficiencies
S Creighton, LB Bloom, MF Goodman - Methods in enzymology, 1995 - Elsevier
Publisher Summary The fidelity of DNA synthesis depends on polymerase-primer template-
nucleotide interactions that can alter fidelity at different locations along a primer-template …
nucleotide interactions that can alter fidelity at different locations along a primer-template …
Ionization of bromouracil and fluorouracil stimulates base mispairing frequencies with guanine
H Yu, R Eritja, LB Bloom, MF Goodman - Journal of Biological Chemistry, 1993 - ASBMB
To test whether ionized base pairs influence polymerase-catalyzed misinsertion rates, we
measured the efficiency of forming 5-bromouracil (B), 5-fluorouracil (F), and thymine base …
measured the efficiency of forming 5-bromouracil (B), 5-fluorouracil (F), and thymine base …
[HTML][HTML] Fidelity of Escherichia coli DNA polymerase III holoenzyme: the effects of β, γ complex processivity proteins and ε proofreading exonuclease on nucleotide …
LB Bloom, X Chen, DK Fygenson, J Turner… - Journal of Biological …, 1997 - ASBMB
The fidelity of Escherichia coli DNA polymerase III (pol III) is measured and the effects of β, γ
processivity and ε proofreading subunits are evaluated using a gel kinetic assay. Pol III …
processivity and ε proofreading subunits are evaluated using a gel kinetic assay. Pol III …
[HTML][HTML] Pre-steady-state kinetic characterization of the AP endonuclease activity of human AP endonuclease 1
RL Maher, LB Bloom - Journal of Biological Chemistry, 2007 - ASBMB
Human AP endonuclease 1 (APE1, REF1) functions within the base excision repair
pathway by catalyzing the hydrolysis of the phosphodiester bond 5 ′ to a baseless sugar (apurinic …
pathway by catalyzing the hydrolysis of the phosphodiester bond 5 ′ to a baseless sugar (apurinic …
Pre-steady-state kinetic analysis of sequence-dependent nucleotide excision by the 3'-exonuclease activity of bacteriophage T4 DNA polymerase
LB Bloom, MR Otto, R Eritja, LJ Reha-Krantz… - Biochemistry, 1994 - ACS Publications
Revised Manuscript Received April 12, 19949 abstract: The effects of local DNA sequence on
the proofreading efficiency of wild-type T 4 DNA polymerase were examined by measuring …
the proofreading efficiency of wild-type T 4 DNA polymerase were examined by measuring …
Base Analog and Neighboring Base Effects on Substrate Specificity of Recombinant Human G: T Mismatch-Specific Thymine DNA− Glycosylase
…, P Gallinari, YZ Xu, MF Goodman, LB Bloom… - Biochemistry, 1996 - ACS Publications
We studied the substrate specificity of the human G:T mismatch-specific thymine glycosylase
that initiates the repair of G:T and G:U base mismatches to G:C base pairs. Such …
that initiates the repair of G:T and G:U base mismatches to G:C base pairs. Such …
[HTML][HTML] Base excision and DNA binding activities of human alkyladenine DNA glycosylase are sensitive to the base paired with a lesion
CW Abner, AY Lau, T Ellenberger, LB Bloom - Journal of Biological …, 2001 - ASBMB
The human alkyladenine DNA glycosylase has a broad substrate specificity, excising a
structurally diverse group of damaged purines from DNA. To more clearly define the structural …
structurally diverse group of damaged purines from DNA. To more clearly define the structural …
Influence of 5'-nearest neighbors on the insertion kinetics of the fluorescent nucleotide analog 2-aminopurine by Klenow fragment
LB Bloom, MR Otto, JM Beechem, MF Goodman - Biochemistry, 1993 - ACS Publications
Revised Manuscript Received August 11, 1993® abstract: The effects of nearest neighbor
interactions between a nucleotide base at the primer 3'-terminus and an incoming …
interactions between a nucleotide base at the primer 3'-terminus and an incoming …
Loading clamps for DNA replication and repair
LB Bloom - DNA repair, 2009 - Elsevier
Sliding clamps and clamp loaders were initially identified as DNA polymerase processivity
factors. Sliding clamps are ring-shaped protein complexes that encircle and slide along …
factors. Sliding clamps are ring-shaped protein complexes that encircle and slide along …