[HTML][HTML] A liquid-to-solid phase transition of the ALS protein FUS accelerated by disease mutation

…, S Saha, TM Franzmann, A Pozniakovski, I Poser… - Cell, 2015 - cell.com
Many proteins contain disordered regions of low-sequence complexity, which cause aging-associated
diseases because they are prone to aggregate. Here, we study FUS, a prion-like …

[PDF][PDF] A human interactome in three quantitative dimensions organized by stoichiometries and abundances

MY Hein, NC Hubner, I Poser, J Cox, N Nagaraj… - Cell, 2015 - cell.com
The organization of a cell emerges from the interactions in protein networks. The interactome
is critically dependent on the strengths of interactions and the cellular abundances of the …

[PDF][PDF] A molecular grammar governing the driving forces for phase separation of prion-like RNA binding proteins

…, R Lemaitre, A Pozniakovsky, D Drechsel, I Poser… - Cell, 2018 - cell.com
Proteins such as FUS phase separate to form liquid-like condensates that can harden into
less dynamic structures. However, how these properties emerge from the collective …

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes

…, J Bulkescher, H Erfle, C Conrad, P Rogers, I Poser… - Nature, 2010 - nature.com
Despite our rapidly growing knowledge about the human genome, we do not know all of the
genes required for some of the most basic functions of life. To start to fill this gap we …

RNA buffers the phase separation behavior of prion-like RNA binding proteins

…, DK Papadopoulos, D Richter, A Pozniakovsky, I Poser… - Science, 2018 - science.org
Prion-like RNA binding proteins (RBPs) such as TDP43 and FUS are largely soluble in the
nucleus but form solid pathological aggregates when mislocalized to the cytoplasm. What …

BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals

I Poser, M Sarov, JRA Hutchins, JK Hériché… - Nature …, 2008 - nature.com
The interpretation of genome sequences requires reliable and standardized methods to assess
protein function at high throughput. Here we describe a fast and reliable pipeline to study …

[PDF][PDF] RNA-induced conformational switching and clustering of G3BP drive stress granule assembly by condensation

…, K Kim, IREA Trussina, J Wang, D Mateju, I Poser… - Cell, 2020 - cell.com
Stressed cells shut down translation, release mRNA molecules from polysomes, and form
stress granules (SGs) via a network of interactions that involve G3BP. Here we focus on the …

Promiscuous interactions and protein disaggregases determine the material state of stress-inducible RNP granules

…, D Mateju, L Malinovska, E Nüske, I Poser… - elife, 2015 - elifesciences.org
10.7554/eLife.06807.001 RNA-protein (RNP) granules have been proposed to assemble by
forming solid RNA/protein aggregates or through phase separation into a liquid RNA/…

Systematic analysis of human protein complexes identifies chromosome segregation proteins

JRA Hutchins, Y Toyoda, B Hegemann, I Poser… - Science, 2010 - science.org
Chromosome segregation and cell division are essential, highly ordered processes that depend
on numerous protein complexes. Results from recent RNA interference screens indicate …

Cortical constriction during abscission involves helices of ESCRT-III–dependent filaments

J Guizetti, L Schermelleh, J Mäntler, S Maar, I Poser… - Science, 2011 - science.org
After partitioning of cytoplasmic contents by cleavage furrow ingression, animal cells remain
connected by an intercellular bridge, which subsequently splits by abscission. Here, we …