Monitoring autophagy by electron microscopy in Mammalian cells

P Ylä‐Anttila, H Vihinen, E Jokitalo, EL Eskelinen - Methods in enzymology, 2009 - Elsevier
… The cells were fixed without treatment (Full), after a 2‐h amino acid starvation (AA‐), and
after a 2‐h amino acid starvation followed by a 2‐h chase in full‐culture medium (AA‐Full). The …

[HTML][HTML] Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells

…, H Ibrahim, A Näätänen, V Chandra, H Vihinen… - Nature …, 2022 - nature.com
… g–h, Proportion SLC18A1 positive cells during S7 culture (g) n = 4–5 and at S7w3 comparing
full S7 medium and S7 medium lacking ZM (h) n = 4, quantified from immunohistochemistry…

3D tomography reveals connections between the phagophore and endoplasmic reticulum

P Ylä-Anttila, H Vihinen, E Jokitalo, EL Eskelinen - Autophagy, 2009 - Taylor & Francis
… amino acid free medium for 1 h to induce autophagy. Atg5 … acid free medium for 1 h, and
immunolabeled with antibodies … in amino acid free medium for 1 h, and double labeled with anti…

Missing-in-metastasis and IRSp53 deform PI(4,5)P2-rich membranes by an inverse BAR domain–like mechanism

…, J Saarikangas, VO Paavilainen, H Vihinen… - The Journal of cell …, 2007 - rupress.org
The actin cytoskeleton plays a fundamental role in various motile and morphogenetic
processes involving membrane dynamics. We show that actin-binding proteins MIM (missing-in-…

[PDF][PDF] Heterochromatin-driven nuclear softening protects the genome against mechanical stress-induced damage

…, DB Whitefield, F Metge, J Boucas, H Vihinen… - Cell, 2020 - cell.com
… (20 mg/ml) in PBS for 1 h at 37 C prior to cell seeding. 600 000 cells per elastomer were
seeded 16 h prior to experiment start, and 3 h before initiation of stretch culture medium was …

[HTML][HTML] Microscopy image browser: a platform for segmentation and analysis of multidimensional datasets

I Belevich, M Joensuu, D Kumar, H Vihinen… - PLoS …, 2016 - journals.plos.org
Understanding the structure–function relationship of cells and organelles in their natural
context requires multidimensional imaging. As techniques for multimodal 3-D imaging have …

Seipin regulates ER–lipid droplet contacts and cargo delivery

VT Salo, I Belevich, S Li, L Karhinen, H Vihinen… - The EMBO …, 2016 - embopress.org
Seipin is an endoplasmic reticulum ( ER ) membrane protein implicated in lipid droplet ( LD
) biogenesis and mutated in severe congenital lipodystrophy ( BSCL 2). Here, we show that …

Biogenesis of the Semliki Forest virus RNA replication complex

P Kujala, A Ikäheimonen, N Ehsani, H Vihinen… - Journal of …, 2001 - Am Soc Microbiol
… Confocal fluorescence images of SFV-infected BHK cells stained for nsP2 at 6 h pi (A),
nsP3 at 6 h pi (B), and nsP4 at 4 h pi (C), double-stained for nsP1 (green) and nsP3 (red) at 4 …

Endoplasmic reticulum remains continuous and undergoes sheet-to-tubule transformation during cell division in mammalian cells

M Puhka, H Vihinen, M Joensuu, E Jokitalo - The Journal of cell biology, 2007 - rupress.org
… A prolonged 2-h puromycin treatment of interphase cells gave similar results, although the
… Interestingly, a 2-h treatment with cycloheximide caused tubulation and perforation of ER …

[PDF][PDF] Seipin facilitates triglyceride flow to lipid droplet and counteracts droplet ripening via endoplasmic reticulum contact

VT Salo, S Li, H Vihinen, M Hölttä-Vuori, A Szkalisity… - Developmental cell, 2019 - cell.com
Seipin is an oligomeric integral endoplasmic reticulum (ER) protein involved in lipid droplet (LD)
biogenesis. To study the role of seipin in LD formation, we relocalized it to the nuclear …