Early on, comparisons of known SPs indicated that they typically have three distinct domains
(Fig. 1): an amino-terminal positively charged region (n-region, 1-5 residues long); a central…

INTRODUCTION Resolving the molecular details of proteome variation in the different tissues
and organs of the human body would greatly increase our knowledge of human biology …

… Unless otherwise indicated, references can be found in von Heijne (1984b). … The c-region
is clearly involved in defining the cleavage site (von Heijne. 1984a); the h-region has been …

Membrane proteins present a hydrophobic surface to the surrounding lipid, whereas portions
protruding into the aqueous milieu expose a polar surface. But how have proteins evolved …

To the Editor: The secretory signal peptide is a ubiquitous proteinsorting signal that targets
its passenger protein for translocation across the endoplasmic reticulum membrane in …

We have developed a new method for the identification of signal peptides and their cleavage
sites based on neural networks trained on separate sets of prokaryotic and eukaryotic …

We describe and validate a new membrane protein topology prediction method, TMHMM,
based on a hidden Markov model. We present a detailed analysis of TMHMM’s performance, …

We describe improvements of the currently most popular method for prediction of classically
secreted proteins, SignalP. SignalP consists of two different predictors based on neural …

A neural network-based tool, TargetP, for large-scale subcellular location prediction of newly
identified proteins has been developed. Using N-terminal sequence information only, it …

A new method for identifying secretory signal sequences and for predicting the site of cleavage
between a signal sequence and the mature exported protein is described. The predictive …