User profiles for Frank Buchholz
Frank BuchholzProfessor of Medical Systems Biology, TU Dresden Verified email at tu-dresden.de Cited by 19039 |
A new logic for DNA engineering using recombination in Escherichia coli
A straightforward way to engineer DNA in E. coli using homologous recombination is
described. The homologous recombination reaction uses RecE and RecT and is transferable …
described. The homologous recombination reaction uses RecE and RecT and is transferable …
Improved properties of FLP recombinase evolved by cycling mutagenesis
The site-specific recombinases FLP and Cre are useful for genomic engineering in many
living systems. Manipulation of their enzymatic properties offers a means to improve their …
living systems. Manipulation of their enzymatic properties offers a means to improve their …
Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice
…, S Weidlich, K Duerschke, F Buchholz… - Disease models & …, 2009 - journals.biologists.com
Tyrosine site-specific recombinases (SSRs) including Cre and FLP are essential tools for
DNA and genome engineering. Cre has long been recognized as the best SSR for genome …
DNA and genome engineering. Cre has long been recognized as the best SSR for genome …
HIV-1 proviral DNA excision using an evolved recombinase
I Sarkar, I Hauber, J Hauber, F Buchholz - Science, 2007 - science.org
HIV-1 integrates into the host chromosome and persists as a provirus flanked by long terminal
repeats (LTRs). To date, treatment regimens primarily target the virus enzymes or virus-…
repeats (LTRs). To date, treatment regimens primarily target the virus enzymes or virus-…
[PDF][PDF] A human interactome in three quantitative dimensions organized by stoichiometries and abundances
…, IA Gak, I Weisswange, J Mansfeld, F Buchholz… - Cell, 2015 - cell.com
The organization of a cell emerges from the interactions in protein networks. The interactome
is critically dependent on the strengths of interactions and the cellular abundances of the …
is critically dependent on the strengths of interactions and the cellular abundances of the …
High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP
Fig. 1 In vivo FLPe-mediated target gene excision. a, b, The ACTB: FLPe transgene contains
a 3.9-kb ACTB fragment inserted into a FLPe expression vector. The indicator transgene …
a 3.9-kb ACTB fragment inserted into a FLPe expression vector. The indicator transgene …
BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals
The interpretation of genome sequences requires reliable and standardized methods to assess
protein function at high throughput. Here we describe a fast and reliable pipeline to study …
protein function at high throughput. Here we describe a fast and reliable pipeline to study …
Minimizing the risk of reporting false positives in large-scale RNAi screens
Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches,
have inherent rates of false positives and negatives. The variability in the standards of …
have inherent rates of false positives and negatives. The variability in the standards of …
Systematic analysis of human protein complexes identifies chromosome segregation proteins
Chromosome segregation and cell division are essential, highly ordered processes that depend
on numerous protein complexes. Results from recent RNA interference screens indicate …
on numerous protein complexes. Results from recent RNA interference screens indicate …
Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I
Segregation of homologous maternal and paternal centromeres to opposite poles during
meiosis I depends on post-replicative crossing over between homologous non-sister …
meiosis I depends on post-replicative crossing over between homologous non-sister …