User profiles for D. Kiyozumi

Daiji Kiyozumi

Research Institute of Environmental Medicine, Nagoya University
Verified email at riem.nagoya-u.ac.jp
Cited by 1283

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice

…, A Isotani, D Kiyozumi… - Proceedings of the …, 2016 - National Acad Sciences
… The number of pups were counted the day after birth or at 10 d after birth. Average litter
sizes are presented as the number of total pups born divided by the number of litters for each …

[HTML][HTML] A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility

D Kiyozumi, K Shimada, M Chalick, C Emori… - Nature …, 2023 - nature.com
… The association rate constant k a , dissociation rate constant k d , and dissociation equilibrium
constant K D for the interaction between purified recombinant NELL2 and NICOL proteins …

Transcriptome-based systematic identification of extracellular matrix proteins

…, Y Oguri, K Shimamoto, D Kiyozumi… - Proceedings of the …, 2008 - National Acad Sciences
… (D) The cell adhesion-promoting activities were examined by incubating MG63, HT1080,
and HeLa cells in 96-well plates containing immobilized GFP-tagged candidate proteins. Cells …

Breakdown of the reciprocal stabilization of QBRICK/Frem1, Fras1, and Frem2 at the basement membrane provokes Fraser syndrome-like defects

D Kiyozumi, N Sugimoto… - Proceedings of the …, 2006 - National Acad Sciences
… Adequate separation of the medium and the cell fraction was monitored by measuring
immunoreactivity to cellular Erk (D). Similarly, 293F cells were transfected with Fras1 expression …

[HTML][HTML] Proteolysis in reproduction: lessons from gene-modified organism studies

D Kiyozumi, M Ikawa - Frontiers in Endocrinology, 2022 - frontiersin.org
… Several proteases have been implicated in D. melanogaster spermatogenesis. In the D.
melanogaster genome, there are five genes paralogous to S. cerevisiae ste24 encoding a type I …

[HTML][HTML] Genetic analysis of fin development in zebrafish identifies furin and hemicentin1 as potential novel fraser syndrome disease genes

…, C Sonntag, K Slanchev, J Kluger, D Kiyozumi… - PLoS …, 2010 - journals.plos.org
… (D,E) Expression of frem1a in the medial fin fold at 24 hpf (D) and pectoral fin at 48 hpf (D-inset).
Expression of frem1b is weaker but can be seen in the tail region, in particular in the …

CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice

…, T Noda, JM Castaneda, D Kiyozumi… - Biology of …, 2019 - academic.oup.com
… (D) Histological analyses of testes and epididymides in Trim17 wild-type and knockout mice.
Scale bars = 50 μm. (E) Morphology of spermatozoa extracted from the cauda epididymides …

[HTML][HTML] Heterozygous Mutations of FREM1 Are Associated with an Increased Risk of Isolated Metopic Craniosynostosis in Humans and Mice

…, J Liu, G Yagnik, K Sekiguchi, D Kiyozumi… - PLoS …, 2011 - journals.plos.org
… The boxed area (yellow) is magnified in (d) highlighting fibrillar staining for Frem1 above
and below the frontal bone (fb) (e) Frem1 protein was also noted diffusely in the suture …

The molecular mechanisms of mammalian sperm maturation regulated by NELL2-ROS1 lumicrine signaling

D Kiyozumi - The Journal of Biochemistry, 2022 - academic.oup.com
In terrestrial vertebrates, spermatozoa generated in the testis are transported through the
reproductive tract toward outside the body. In addition to as the pathway of sperm transport, the …

NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility

D Kiyozumi, T Noda, R Yamaguchi, T Tobita… - Science, 2020 - science.org
Kiyozumi et al. identified NELL2 as a testicular luminal protein that binds to its receptor,
ROS1, on the luminal epididymis surface and induces epididymal differentiation (see the …