… (C) RNase protection assays of Upf1 and Upf1DCH in presence of different nucleotides. …
(C) Crystal structure of yeast Upf1 in complex with eight ribonucleotides of a U15 RNA and ADP:…

… Samples were heated to 95C for 5 min and then subjected to 36 cycles of … at 95C for 30 s,
annealing at 59C for 30 s, and extension at 72C for 30 s, followed by a 7-min extension at 72C …

The Na + /I − symporter (NIS) is a key plasma membrane protein that mediates active I −
uptake in the thyroid, lactating breast, and other tissues with an electrogenic stoichiometry of 2 …

The DEAD-box protein DBP5 is essential for mRNA export in both yeast and humans. It binds
RNA and is concentrated and locally activated at the cytoplasmic side of the nuclear pore …

… As a control, we generated a plasmid with a C-terminal truncation (Not11–1696) that eliminates
an essential portion of Not1 involved in the interaction with Not2/3/5 (of unknown function…

… The C-terminal region (Rrp6 C ) wraps around the outer surface of Exo-9 contacting Mtr3,
Rrp43 and Csl4. Rrp6 C contributes the strongest interaction of Rrp6 to the exosome both in …

… Mutation of conserved residues within Rrp6 and Mtr4 at the structural interface disrupts their
interaction and inhibits growth of strains expressing a C‐terminal GFP fusion of Mtr4. These …

… (RRM) domain that mediates the interaction with the C-terminal MIF4G (middle portion of
eIF4G) domain of UPF2 (18). At the C terminus, human UPF3 contains a region that has been …

… 4 C for 2 h followed by 20 C for 15 min. The excitation and emission wavelengths were 485
and 535 nm, respectively. Each titration point was measured three times using ten readings …

… between IFT27, Rab8 and Rab11, the C-termini of the proteins are highly divergent. A typical
hallmark of Rab proteins is the presence of a C-terminal prenylation motif consisting of one …