Fused in sarcoma (FUS) is a nuclear protein that carries a proline‐tyrosine nuclear localization
signal (PY‐NLS) and is imported into the nucleus via Transportin (TRN). Defects in …

… To further address functionality of the predicted NESs, we fused them to the C-terminus of
EGFP, which is localized in both the nucleus and the cytoplasm, with a predominant nuclear …

… (c) APPPS1 transgenic animals injected with APPPS1 transgenic brain homogenates mixed
… was centrifuged for 5 min (at 3000g, 4 C) and the supernatant was stored at −80 C until use. …

… One litre of M9 minimal medium was inoculated, cultures were grown for 2 days at 25 C,
diluted to OD 1.0 and induced with 0.5 mM IPTG for either 6 h at 25 C or 14 h at 19 C. Cells were …

Genetic factors undoubtedly affect the development of congenital heart disease (CHD) but
still remain ill defined. We sought to identify genetic risk factors associated with CHD and to …

… was diminished and disappeared over time (Figure 2A,C). The same phenomenon was
observed in APP23 transgenic mice (Figure 2B,C). To account for the differences in the amount …

… B and C, Representative plots (B) and statistical analysis (C) of contractile force in HLHS and
control patches over 24 days of culture. In (C), data are mean±SEM of serial measurements …

… categories: n > C (more protein in the nucleus than the cytoplasm), n = C (equal distribution
of the reporter protein between nucleus and cytoplasm), and n < C (more reporter protein in …

… 2.5% of the cDNA were used for a SYBR Green real-time PCR reaction (40 cycles with 15 s
at 95 C and 60 s at 65 C) on a Bio-Rad IQ5 machine using described primers for Nicalin and …

Amyloid-β (Aβ) plaque deposition plays a central role in the pathogenesis of Alzheimer’s
disease (AD). Post-mortem analysis of plaque development in mouse models of AD revealed …